Unknown,Transcriptomics,Genomics,Proteomics

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BI Human Reference Epigenome Mapping Project: Characterization of chromatin modification by ChIP-Seq in human subject

ABSTRACT: Genome scale characterization of chromatin modification, RNA expression, and cytosine methylation in a diverse panel of primary human cells and tissues, stem cells, and iPS cells derived from deidentified human subjects **************** For data usage terms and conditions, please refer to: http://www.nida.nih.gov/about/roadmap/epigenomics/data_access_policies.html **************** BI Human Reference Epigenome Mapping Project - samples and experiments will continue to be added to this study over the course of the project. EDACC Genboree Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSTUDY%2FEDACC.3212

ORGANISM(S): Homo sapiens

SUBMITTER: BROAD INSTITUTE

PROVIDER: E-GEOD-25249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Analysis of transcriptional regulation in human cells has implicated a large number of promoter-specific DNA-binding proteins that regulate transcription via diverse mechanisms. In some cases, these DNA-sequence-specific factors associate with intermediaries that orchestrate interactions with the chromatin-modifying enzymes. One such intermediary is HCF-1 (host-cell factor-1; HCFC1). HCF-1, first identified for its involvement in herpes-simplex virus transcription and subsequently shown to be an important cell-cycle regulator, has a poorly defined role in genome-wide transcriptional regulation. We show here, by chromatin immunoprecipitation followed by high-throughput sequence analysis (ChIP-seq), that HCF-1 is a major transcriptional start site associated factor, whose promoter association correlates positively with transcriptional activity. Thus, in HeLa cells HCF-1 is observed on 5400 generally active CpG-island promoters. Examination of the DNA sequences underlying the HCF-1-binding sites revealed three sequence motifs associated with the binding of (i) ZNF143 and Ronin/THAP11, (ii) GABP, and (iii) YY1 sequence-specific transcription factors. Subsequent ChIP-seq analysis of these four transcription factors revealed a large co-association of HCF-1 with these four transcription factors at approximately 90% of HCF-1-bound promoters. These studies suggest that a relatively small number of transcription factors -- some (ZNF143 and Ronin/THAP11) in novel combinations -- play a major role in HeLa-cell transcriptional regulation in association with HCF-1. This experiment includes the sequencing data of material obtained from chromatin immunoprecipitation using antibodies against HCFC1(antibodies against the C-subunit and the N-subunit), PolII(antibody against the RPB2 subunit), H3K36ME3 and H3K4Me3 histone modifications, ZNF143, THAP11, GABPalpha and YY1. It also includes control data of the Input material. All of them were done using HeLa cycling cells.

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BI Human Reference Epigenome Mapping Project: Characterization of chromatin modification by ChIP-Seq in human subject

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