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Expression data from third instar larval eye discs

ABSTRACT: Expression of dE2F1 induces proliferation and apoptosis. We sought to perform an unbiased analysis of the effect of co-expression of miR-11 We used microarrays to identify differentially regulated genes following expression of dE2F1 and/or dme-miR-11 using the GMR-Gal4 driver Drosophila third instar larvae were selected for RNA extraction and hybridization on Affymetrix microarrays. The GMR-Gal4 driver induces expression posterior to the morphogenetic furrow. We compared the gene expression profiles following induction of expression of dE2F1 + dDP and/or dme-miR-11.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Mary Truscott

PROVIDER: E-GEOD-25267 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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mir-11 limits the proapoptotic function of its host gene, dE2f1.

Truscott Mary M Islam Abul B M M K AB López-Bigas Núria N Frolov Maxim V MV

Genes & development 20110819 17

The E2F family of transcription factors regulates the expression of both genes associated with cell proliferation and genes that regulate cell death. The net outcome is dependent on cellular context and tissue environment. The mir-11 gene is located in the last intron of the Drosophila E2F1 homolog gene dE2f1, and its expression parallels that of dE2f1. Here, we investigated the role of miR-11 and found that miR-11 specifically modulated the proapoptotic function of its host gene, dE2f1. A mir-1 ...[more]

PMID: 21856777

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Expression data from third instar larval eye discs

Project description:Expression of dE2F1 induces proliferation and apoptosis. We sought to perform an unbiased analysis of the effect of co-expression of miR-11 We used microarrays to identify differentially regulated genes following expression of dE2F1 and/or dme-miR-11 using the GMR-Gal4 driver

2011-09-01 | GSE25267 | GEO

The Drosophila functional Smad suppressing element fuss, a homologue of the human Skor genes, retains prooncogenic properties of the Ski/Sno family

Project description:To detect dysregulated genes upon overexpression of the functional smad suppressing element (fuss) in Drosophila melanogaster, we overexpressed fuss with the GMR-Gal4 line in developing eye discs and with the nub-Gal4 driverline in developing wing discs. The offspring of crossings of the driver lines GMR-Gal4 or nub-Gal4 with w1118 were used as controls. Libraries were generated from total RNA and sequenced on a Illumina HiSeq 1000. Via differential gene expression analysis we discovered the genes which are dysregulated by fuss overexpression compared to controls.

2022-01-05 | GSE165738 | GEO

Expression data from Control and Yorkie overexpression pupal eye discs

2011-12-20 | GSE26700 | GEO

HEXIM knockdown triggers apoptosis-induced proliferation and deregulates Hedgehog signaling

Project description:We address the function of HEXIM, an inhibitor of the general transcriptional elongation regulator P-TEFb which regulates the transcriptional status of many developmental genes, during Drosophila development. We showed that HEXIM knockdown mutants display organs development failure. In the wing disc, it induces apoptosis and affects Hh signaling. The continuous death of proliferative cells is compensated by apoptosis-induced cell proliferation, in a manner similar to that of differentiated cells, together with high levels of Hh and Ci. We completed this analysis with microarrays to characterize the molecular phenotype of HEXIM knockdown during eye differentiation. HEXIM knockdown during eye differentiation was carried out with a UAS-Hexim-RNAi strain crossed with a GMR-Gal4 driver strain (Bloomington stock center)). Adult GMR>RNAi Hexim flies display slightly smaller, rough and often necrosed eyes. Total RNA were collected from isolated heads. We then used microarray to compare the transcriptome of HEXIM knockdown mutant with that of wild type flies.

2014-07-31 | E-GEOD-54590 | biostudies-arrayexpress

micro RNA (miR) expression profile of developing fly eye in different Ago-1 expressing condition

Project description:As miRs are one of the crucial regulators of various cellular processes, our interest was to see whether any change in their expression occurs under Ago-1 overexpression. miRNA microarray analysis was carried out using total RNA extracted from control (GMR-GAL4), Ago-1 overexpressed (UAS Ago-1/GMR GAL4) and Ago-1 mutant (Ago-1^72/ Ago-1^45) adult eye. Several miRNAs were upregulated and several showed down regulation. miRs, which has shown significant fold change in there expression, were selected for further study.

2018-02-14 | GSE102820 | GEO

miRNA expression of lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP

Project description:To investigate the difference of miRNA expression between lung cancer cell A549 and its DDP-resistant cell strain A549/DDP, we have employed miRNA microarray expression to discover the difference expression of miRNAs of A549 cells and A549/DDP. We conducted RT-qPCR to examine the expression levels of top differential expressed miRNAs, namely, miR-197-5p, miR-4443, miR-642a-3p, miR-27b-3p and miR-100-5p, confirming low variability between two methods. The A549/DDP was established from A549 in our laboratory, by exposing A549 to gradually increasing DDP concentrations, until the final concentration at 1Î¼g/ml. To avoid the influence of drug to the A549/DDP cells, they were cultured in a drug-free medium for at least two weeks before gene expression analysis. miRNA expression of A549 and A549/DDP was then analzyed.

2016-08-16 | E-GEOD-85603 | biostudies-arrayexpress

miRNA expression of exosomes derived from lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP

Project description:To investigate the difference of miRNA expression in exosomes derived from A549 cells and its DDP-resistant cell strain A549/DDP, we have employed miRNA microarray expression to discover the difference expression of miRNAs in exosomes derived from A549 and A549/DDP. We conducted RT-qPCR to examine the expression levels of top differential expressed miRNAs, namely, miR-197-5p, miR-4443, miR-642a-3p, miR-27b-3p and miR-100-5p, confirming low variability between two methods. The A549/DDP was established from A549 in our laboratory, by exposing A549 to gradually increasing DDP concentrations, until the final concentration at 1Î¼g/ml. To avoid the influence of drug to the A549/DDP cells, they were cultured in a drug-free medium for at least two weeks before gene expression analysis. After being incubated for 48-72h, the culture medium of cells was harvested. Exosomes were isolated by ultracentrifugation. And miRNA expression of exosomes derived from A549 and A549/DDP was then analzyed.

2016-08-16 | E-GEOD-85604 | biostudies-arrayexpress

miRNA expression of lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP

2016-08-16 | GSE85603 | GEO

miRNA expression of exosomes derived from lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP

2016-08-16 | GSE85604 | GEO

Expression data from Control and Yorkie overexpression pupal eye discs

Project description:To probe mechanistic determinants of Yki function in mitochondrial biogenesis, we conducted a genome-wide microarray experiment, and specifically compared expression patterns of genes from control (GMR gal4) and Yorkie over-expressing (GMR gal4; UAS yki) pupal eye discs. The above mentioned genotypes were grown at 29 deg C and at about 40 hrs after pupariation, the pupal eye discs were dissected. RNA was extracted from the pupal eye discs, purified and used to generate microarray probes that were hybridized to the Drosophila genome 2 arrays (Affymetrix). The Gene Chip Operating system (Affymetrix) and dCHIP program (Harvard University) were used to generate pairwise comparisons between the transcription profiles of control and Yorkie over-expressing discs.

2011-12-20 | E-GEOD-26700 | biostudies-arrayexpress

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