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Affy_sunyfuel_drought_sunflower-Sunyfuel

ABSTRACT: affy_sunyfuel_drought_sunflower - Drought is the main cause or crop loss around the globe and it is expected to be more so in years to come. Helianthus genus comprises species that are able to grow in harsh environments suffering from severe water shortage. Cultivated H.annuus, i.e. sunflower, is a highly valuable oil crop due to, among other features, its ability to grow under low input conditions. The aim of our project is to unravel the transcriptional regulation underpinning drought tolerance in sunflower, with the ultimate goal of improving crop yield. Ninety six individual pots were arranged in six blocks of 16 plants each, which were sown in individual pots. Each block contained 2 plants of 8 different genotypes, one of which was used as control and the other one as treated plant. Plants within each block were randomly arranged. At the right developmental stage, treated plants were water-deprived and their water status was monitored as compared to the untreated control. Three out of the six blocks were harvested on a given date, so water stress among genotypes varied. The plants in the other three blocks were harvested when the treated plant reached a given water stress. 96 arrays - SUNFLOWER; treated vs untreated comparison

ORGANISM(S): Helianthus annuus

SUBMITTER: sandrine balzergue

PROVIDER: E-GEOD-25719 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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affy_sunyfuel_drought_sunflower-Sunyfuel

Project description:affy_sunyfuel_drought_sunflower - Drought is the main cause or crop loss around the globe and it is expected to be more so in years to come. Helianthus genus comprises species that are able to grow in harsh environments suffering from severe water shortage. Cultivated H.annuus, i.e. sunflower, is a highly valuable oil crop due to, among other features, its ability to grow under low input conditions. The aim of our project is to unravel the transcriptional regulation underpinning drought tolerance in sunflower, with the ultimate goal of improving crop yield. Ninety six individual pots were arranged in six blocks of 16 plants each, which were sown in individual pots. Each block contained 2 plants of 8 different genotypes, one of which was used as control and the other one as treated plant. Plants within each block were randomly arranged. At the right developmental stage, treated plants were water-deprived and their water status was monitored as compared to the untreated control. Three out of the six blocks were harvested on a given date, so water stress among genotypes varied. The plants in the other three blocks were harvested when the treated plant reached a given water stress.

2012-07-10 | GSE25719 | GEO

affy_sunflower_2010_13-ROS and hormones in sunflower seed dormancy.

Project description:affy_sunflower_2010_13 - affy_sunflower_2010_13 - It concerns the interaction between ROS and hormones in dormancy release in sunflower seeds. ABA is responsible for dormancy maintenance, while GA and ethylene promote seed germination. Based on our results, ROS could represent good candidate to shift from a hormone signalling to another determining the dormancy state in sunflower seeds.-We aim to understand the mechanisms controlling sunflower seed dormancy at the transcriptomic level, by the application of treatments which maintain dormancy as ABA, or alleviate dormancy as ROS and ethylene. Transcripts comparison will be performed between dormant and non-dormant sunflower embryo imbibed 24h on water, on ABA, on methylviologen, a pro-oxidant compound or on ethylene. 12 arrays - SUNFLOWER; treated vs untreated comparison

2013-08-01 | E-GEOD-44165 | biostudies-arrayexpress

water stress-Identification of targets of the transcription factor hahb-4 in relation to abiotic stress

Project description:rs04-09_hahb4 - water stress - We want to know which genes are over-expressed or down regulated in Arabidopsis plants transformed with the sunflower hahb-4 homeodomain transcription factor with respect to non transformed ones in control and water stress conditions. This gene, hahb4, confers drought tolerance to transgenic Arabidopsis plants. - Overexpression of the hahb4 gene vs vector transformed control Keywords: gene knock in (transgenic),treated vs untreated comparison

2007-08-31 | GSE8878 | GEO

affy_tour_2010_21-Assessing drought stress transcriptomics in Helianthus annus in field cultures

Project description:affy_tour_2010_21 - affy_tour_2010_21 - The aim of this project is to assess the possibility of implementing transcriptomic studies on sunflower plants grown in field assays. Data obtained from plants under drought in the glasshouse have already obtained. We intend to use these results as a template and see if the field offers the possibility of carrying these studies. In order to do so, treatment-effect as well as intra and inter-plot variability will be assessed.-Plants were grown in the field. The assay was arranged in plots with one genotype per plot. The assay was divided in two identically seized parts, containing the same number of plots. Starting from the capitule, flower # -3 was harvested in every plant. In some cases, several plants from the same plot were harvested. In other, plants from different plots were harvested. We expect that this sampling will allow us to assess intra- as well as inter-plot variability in the analysis of drought-driven gene expression modulation. 24 arrays - SUNFLOWER; treated vs untreated comparison

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Project description:affy_strigolactone_sunflower - affy_strigolactone_sunflower - Abiotic stress and more specifically drought is the major limiting factor for sunflower production. Sunflower response to drought includes root plasticity to adapt to water availability and reach soil water. We identified genotype-specific responses of root architecture to strigolactone application. This experiment aims at identifying strigolactone responsive pathways in 6 genotypes in order to better understand molecular control of root development in sunflower and therefore its response to drought. The ultimate goal will be to improve sunflower breeding through selection of key drought response genes.-The experiment consisted of 2 repeats of four 12-day-old-plantlets of 6 sunflower genotype SF193 (INRA code: XRQ), SF326 (INRA code: PSC8), SF056 (INRA code: FU), SF306 (INRA code: PAZ2), SF302 (INRA code: PAC2), SF268 (INRA code: RHA266), grown in growth chamber conditions and submitted to a 24-hour-treatment of 100 nM strigolactone analogue rac-GR24 (Chiralix, Nijmegen, Netherland) or not. Growth conditions were 14h light at 23Â°C and 10h night at 20Â°C under fluorescent bulbs. Plants were grown in hydroponic boxes containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). The entire root systems were harvested 4 hours after light onset and frozen immediately in liquid nitrogen. 24 arrays - SUNFLOWER; treated vs untreated comparison

2013-05-06 | E-GEOD-25888 | biostudies-arrayexpress

Transcription profiling of Arabidopsis under water stress was applied to transgenic and non transformed three weeks old arabidopsis plants control and overexpressing line hahb-4 in relation to abiotic stress

Project description:We want to know which genes are overexpressed or down regulated in Arabidopsis plants transformed with the sunflower hahb-4 homeodomain transcription factor with respect to non transformed ones in control and water stress conditions. This gene, hahb4, confers drought tolerance to transgenic arabidopsis plants. Aa water stress was applied to transgenic and non transformed three weeks old arabidopsis plants control and overexpressing line

2005-06-13 | E-MEXP-394 | biostudies-arrayexpress

water stress-Identification of targets of the transcription factor hahb-4 in relation to abiotic stress

2007-08-30 | E-GEOD-8878 | biostudies-arrayexpress

affy_strigolactone_sunflower-Identification of Strigolactone -regulated genes in sunflower

Project description:affy_strigolactone_sunflower - affy_strigolactone_sunflower - Abiotic stress and more specifically drought is the major limiting factor for sunflower production. Sunflower response to drought includes root plasticity to adapt to water availability and reach soil water. We identified genotype-specific responses of root architecture to strigolactone application. This experiment aims at identifying strigolactone responsive pathways in 6 genotypes in order to better understand molecular control of root development in sunflower and therefore its response to drought. The ultimate goal will be to improve sunflower breeding through selection of key drought response genes.-The experiment consisted of 2 repeats of four 12-day-old-plantlets of 6 sunflower genotype SF193 (INRA code: XRQ), SF326 (INRA code: PSC8), SF056 (INRA code: FU), SF306 (INRA code: PAZ2), SF302 (INRA code: PAC2), SF268 (INRA code: RHA266), grown in growth chamber conditions and submitted to a 24-hour-treatment of 100 nM strigolactone analogue rac-GR24 (Chiralix, Nijmegen, Netherland) or not. Growth conditions were 14h light at 23°C and 10h night at 20°C under fluorescent bulbs. Plants were grown in hydroponic boxes containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). The entire root systems were harvested 4 hours after light onset and frozen immediately in liquid nitrogen.

2013-05-06 | GSE25888 | GEO

affy_sunflower_2011_02-Effect of frost on sunflower

Project description:affy_sunflower_2011_02 - affy_sunflower_2011_02 - The early sowing constitutes an alternative strategy to avoid drought occurring during flowering and post-flowering periods and responsible for decrease in sunflower production. In French cropping system, early sowing is associated to low temperature period and frost during first development stages in sunflower. Knowledge about metabolism of frost acclimation must be performed to supply tools for breeding programs in sunflower. The aim of our experiment is to unravel the transcriptional regulation underpinning frost tolerance in sunflower-5 genotypes of sunflower were grown in a growth chamber 1 (23Â°C day/18Â°C night, 63% air humidity, 14 hours day photoperiod). At the stage of 6 leaves well-developed, 12 plants of each genotype were subjected to cold acclimation (+4Â°C during 2 days) in another growth chamber 2. Then, these plants were subjected to 2 nights at -3Â°C (frost treatment). Chlorophylle fluorescence, Osmotic potential, Relative electrolyte leakage were then determined in the following conditions : - 6th October on 6 plants X 5 genotypes from growth chamber 1 (C1) - 6th October on 6 plants X 5 genotypes from growth chamber 2 (S1) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (C2) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (S2) 12 arrays - SUNFLOWER; treated vs untreated comparison

2013-09-01 | E-GEOD-45586 | biostudies-arrayexpress

affy_sunflower_2011_02-Effect of frost on sunflower

Project description:affy_sunflower_2011_02 - affy_sunflower_2011_02 - The early sowing constitutes an alternative strategy to avoid drought occurring during flowering and post-flowering periods and responsible for decrease in sunflower production. In French cropping system, early sowing is associated to low temperature period and frost during first development stages in sunflower. Knowledge about metabolism of frost acclimation must be performed to supply tools for breeding programs in sunflower. The aim of our experiment is to unravel the transcriptional regulation underpinning frost tolerance in sunflower-5 genotypes of sunflower were grown in a growth chamber 1 (23°C day/18°C night, 63% air humidity, 14 hours day photoperiod). At the stage of 6 leaves well-developed, 12 plants of each genotype were subjected to cold acclimation (+4°C during 2 days) in another growth chamber 2. Then, these plants were subjected to 2 nights at -3°C (frost treatment). Chlorophylle fluorescence, Osmotic potential, Relative electrolyte leakage were then determined in the following conditions : - 6th October on 6 plants X 5 genotypes from growth chamber 1 (C1) - 6th October on 6 plants X 5 genotypes from growth chamber 2 (S1) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (C2) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (S2)

2013-09-01 | GSE45586 | GEO

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