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Delineation of AR oncogenic functions in hepatocellular carcinoma

ABSTRACT: The goal of this study was to delineate the important AR direct target genes that mediate the oncogenic properties of AR in HCC. The AR direct target genes in two HCC cell lines were identified by chromatin immunoprecipitation microarray (ChIP-chip) analysis and later confirmed by independent ChIP-PCR. The functions of the target genes were further examined. Two human HCC cell lines, Huh7 and PLC/PRF/5 (PLC5), were grown in DMEM supplemented with 10% fetal bovine serum. ChIP assays were performed using anti-AR antibody. The immunoprecipitated and input DNA were used to probe the Agilent human ChIP-chip arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Hai Feng

PROVIDER: E-GEOD-25884 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cell cycle-related kinase is a direct androgen receptor-regulated gene that drives β-catenin/T cell factor-dependent hepatocarcinogenesis.

Feng Hai H Cheng Alfred S L AS Tsang Daisy P DP Li May S MS Go Minnie Y MY Cheung Yue S YS Zhao Gui-jun GJ Ng Samuel S SS Lin Marie C MC Yu Jun J Lai Paul B PB To Ka F KF Sung Joseph J Y JJ

The Journal of clinical investigation 20110711 8

Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide. It is more prevalent in men than women. Related to this, recent genetic studies have revealed a causal role for androgen receptor (AR) in hepatocarcinogenesis, but the underlying molecular mechanism remains unclear. Here, we used genome-wide location and functional analyses to identify a critical mediator of AR signaling - cell cycle-related kinase (CCRK) - that drives hepatocarcinogenesis via a signaling pathway dependent ...[more]

PMID: 21747169

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