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Expression data of WT and ced1 under osmotic stress

ABSTRACT: ced1 mutant has reduced expression of NCED3 in response to osmotic stress (polyethylene glycol) treatments compared to the wild type. Other ABA biosynthesis genes are also greatly reduced in ced1 under osmotic stress. We used microarrays to detail the global programme of gene expression under osmotic stress treatment and identified distinct classes of up/down-regulated genes in the stress pathway. Arabidopsis seedlings were selected at 16 days with 6hr PEG treatment for RNA extraction and hybridization on Affymetrix microarrays. From Norther data we have known some of well-known stress responsive gene were significantly changed. We sought to obtain whole gene expression to analysis which genes were affected in the osmotic stress pathway.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: zhenyu wang 

PROVIDER: E-GEOD-25889 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The plant cuticle is required for osmotic stress regulation of abscisic acid biosynthesis and osmotic stress tolerance in Arabidopsis.

Wang Zhen-Yu ZY   Xiong Liming L   Li Wenbo W   Zhu Jian-Kang JK   Zhu Jianhua J  

The Plant cell 20110524 5

Osmotic stress activates the biosynthesis of abscisic acid (ABA). One major step in ABA biosynthesis is the carotenoid cleavage catalyzed by a 9-cis epoxycarotenoid dioxygenase (NCED). To understand the mechanism for osmotic stress activation of ABA biosynthesis, we screened for Arabidopsis thaliana mutants that failed to induce the NCED3 gene expression in response to osmotic stress treatments. The ced1 (for 9-cis epoxycarotenoid dioxygenase defective 1) mutant isolated in this study showed mar  ...[more]

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