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LIM domain binding protein 1 regulates a transcriptional program essential for hematopoietic stem cell maintenance

ABSTRACT: We used ChIP-Seq to map Ldb1, Scl and Gata2 binding sites in mouse hematopoietic progenitor cells (HPCs). Together with functional studies using conventional and conditional Ldb1 deficient mouse models and bioinformatics analysis, we systematically determined a transcriptional program controlled by Ldb1 complexes in HSC maintenance. To evaluate the role of Ldb1 in hematopoietic stem cell maintenance.

ORGANISM(S): Mus musculus

SUBMITTER: Raja Jothi

PROVIDER: E-GEOD-26031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nuclear adaptor Ldb1 regulates a transcriptional program essential for the maintenance of hematopoietic stem cells.

Li LiQi L Jothi Raja R Cui Kairong K Lee Jan Y JY Cohen Tsadok T Gorivodsky Marat M Tzchori Itai I Zhao Yangu Y Hayes Sandra M SM Bresnick Emery H EH Zhao Keji K Westphal Heiner H Love Paul E PE

Nature immunology 20101226 2

The nuclear adaptor Ldb1 functions as a core component of multiprotein transcription complexes that regulate differentiation in diverse cell types. In the hematopoietic lineage, Ldb1 forms a complex with the non-DNA-binding adaptor Lmo2 and the transcription factors E2A, Scl and GATA-1 (or GATA-2). Here we demonstrate a critical and continuous requirement for Ldb1 in the maintenance of both fetal and adult mouse hematopoietic stem cells (HSCs). Deletion of Ldb1 in hematopoietic progenitors resul ...[more]

PMID: 21186366

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Ldb1-nucleated transcription complexes function as primary mediators of erythroid gene activation

Project description:We used ChIP-Seq to map Ldb1, Scl and Gata1 binding sites in mouse total bone marrow cells. Together with functional studies comparing gene expression in Murine Erythroleukemia (MEL) cells expressing Ldb1 shRNA or control shRNA and bioinformatics analysis, we systematically determined the transcriptional program controlled by Ldb1 complexes in erythropoiesis. This represents the ChIP-Seq component of the study only To evaluate the role of Ldb1complexes in erythroid gene activation

2013-04-29 | E-GEOD-42843 | biostudies-arrayexpress

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2013-02-09 | E-GEOD-43042 | biostudies-arrayexpress

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2013-02-09 | E-GEOD-43041 | biostudies-arrayexpress

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2011-01-04 | GSE26031 | GEO

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2010-12-10 | E-GEOD-24933 | biostudies-arrayexpress

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