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Mut-16 and other mutator-class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans

ABSTRACT: Argonaute-associated siRNAs and Piwi-associated piRNAs have overlapping roles in silencing mobile genetic elements in animals. In C. elegans, mutator-class (mut) genes mediate siRNA-guided repression of transposons as well as exogenous RNA-directed gene silencing (RNAi), but their roles in endogenous RNA silencing pathways are not well understood. To characterize the endogenous small RNAs dependent on mutator-class genes, small RNA populations from a null allele of mut-16, as well as a regulatory mut-16(mg461) allele that disables only somatic RNAi, were subjected to deep sequencing. Small RNA analysis in wild type and mut-16 mutant C. elegans strains

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Taiowa Montgomery

PROVIDER: E-GEOD-26165 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans.

Zhang Chi C Montgomery Taiowa A TA Gabel Harrison W HW Fischer Sylvia E J SE Phillips Carolyn M CM Fahlgren Noah N Sullivan Christopher M CM Carrington James C JC Ruvkun Gary G

Proceedings of the National Academy of Sciences of the United States of America 20110118 4

Argonaute-associated siRNAs and Piwi-associated piRNAs have overlapping roles in silencing mobile genetic elements in animals. In Caenorhabditis elegans, mutator (mut) class genes mediate siRNA-guided repression of transposons as well as exogenous RNAi, but their roles in endogenous RNA silencing pathways are not well-understood. To characterize the endogenous small RNAs dependent on mut class genes, small RNA populations from a null allele of mut-16 as well as a regulatory mut-16(mg461) allele ...[more]

PMID: 21245313

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MUT-14 and SMUT-1 DEAD box RNA helicases have overlapping roles in germline RNAi and endogenous siRNA formation in C. elegans

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mut-16 and other mutator-class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans

Project description:Argonaute-associated siRNAs and Piwi-associated piRNAs have overlapping roles in silencing mobile genetic elements in animals. In C. elegans, mutator-class (mut) genes mediate siRNA-guided repression of transposons as well as exogenous RNA-directed gene silencing (RNAi), but their roles in endogenous RNA silencing pathways are not well understood. To characterize the endogenous small RNAs dependent on mutator-class genes, small RNA populations from a null allele of mut-16, as well as a regulatory mut-16(mg461) allele that disables only somatic RNAi, were subjected to deep sequencing.

2011-02-01 | GSE26165 | GEO

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Caenorhabditis elegans

Project description:mut-16 and other mutator-class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans

| PRJNA135287 | ENA

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