Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Differential expression of keratin genes in regenerating digit of C57Bl/6 versus MRL/MpJ mice

ABSTRACT: Expression of genes encoding multiple keratin- and keratin-associated proteins varies between C57BL/6 and MRL/MpJ (superhealer) mice following digit amputation. We characterized changes in gene expression by microarray analysis of regenerating digits in these mice at various timepoints following surgical amputation. These changes were validated by quantitative rtPCR analyses. In this dataset, we include the expression data obtained from regenerating mouse digits following surgical amputation as well unamputated digits (total 24 samples). These data are used to obtain genes that are differentially expressed between the two groups of digits.

ORGANISM(S): Mus musculus

SUBMITTER: Chia-Ho Cheng

PROVIDER: E-GEOD-26227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Keratin gene expression profiles after digit amputation in C57BL/6 vs. regenerative MRL mice imply an early regenerative keratinocyte activated-like state.

Cheng Chia-Ho CH Leferovich John J Zhang Xiang-Ming XM Bedelbaeva Khamilia K Gourevitch Dmitri D Hatcher Cathy J CJ Basson Craig T CT Heber-Katz Ellen E Marx Kenneth A KA

Physiological genomics 20130319 11

Mouse strains C57BL/6 (B6) and MRL were studied by whole mouse genome chip microarray analyses of RNA isolated from amputation sites at different times pre- and postamputation at the midsecond phalange of the middle digit. Many keratin genes were highly differentially expressed. All keratin genes were placed into three temporal response classes determined by injury/preinjury ratios. One class, containing only Krt6 and Krt16, were uniquely expressed relative to the other two classes and exhibited ...[more]

PMID: 23512742

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Dataset Information

Differential expression of keratin genes in regenerating digit of C57Bl/6 versus MRL/MpJ mice

Publications

Keratin gene expression profiles after digit amputation in C57BL/6 vs. regenerative MRL mice imply an early regenerative keratinocyte activated-like state.

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