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ChIP-seq for CTCF and Rad21 in Rag1M-bM-^HM-^R/M-bM-^HM-^R pro-B cells

ABSTRACT: Genome-wide ChIP data of CTCF and Rad21 binding in Rag1M-bM-^HM-^R/M-bM-^HM-^R pro-B cells CTCF and Rad21 binding in Rag1M-bM-^HM-^R/M-bM-^HM-^R pro-B

ORGANISM(S): Mus musculus

SUBMITTER: Ali Torkamani

PROVIDER: E-GEOD-26257 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense transcription in pro-B cells.

Degner Stephanie C SC Verma-Gaur Jiyoti J Wong Timothy P TP Bossen Claudia C Iverson G Michael GM Torkamani Ali A Vettermann Christian C Lin Yin C YC Ju Zhongliang Z Schulz Danae D Murre Caroline S CS Birshtein Barbara K BK Schork Nicholas J NJ Schlissel Mark S MS Riblet Roy R Murre Cornelis C Feeney Ann J AJ

Proceedings of the National Academy of Sciences of the United States of America 20110523 23

Compaction and looping of the ~2.5-Mb Igh locus during V(D)J rearrangement is essential to allow all V(H) genes to be brought in proximity with D(H)-J(H) segments to create a diverse antibody repertoire, but the proteins directly responsible for this are unknown. Because CCCTC-binding factor (CTCF) has been demonstrated to be involved in long-range chromosomal interactions, we hypothesized that CTCF may promote the contraction of the Igh locus. ChIP sequencing was performed on pro-B cells, revea ...[more]

PMID: 21606361

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