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Gene expression from bone-marrow derived macrophages.

ABSTRACT: Gene expression from WT and NFAT5 KO primary macrophage cultures. Keywords: Bone-marrow derived macrophages. We analyzed 4 arrays from each condition: unstimulated WT BMDMs, LPS stimulated WT BMDMs, unstimulated KO BMDMs, LPS stimulated KO BMDMs.

ORGANISM(S): Mus musculus

SUBMITTER: Cristina Lopez-Rodriguez

PROVIDER: E-GEOD-26343 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression induced by Toll-like receptors in macrophages requires the transcription factor NFAT5.

Buxadé Maria M Lunazzi Giulia G Minguillón Jordi J Iborra Salvador S Berga-Bolaños Rosa R Del Val Margarita M Aramburu José J López-Rodríguez Cristina C

The Journal of experimental medicine 20120206 2

Toll-like receptors (TLRs) engage networks of transcriptional regulators to induce genes essential for antimicrobial immunity. We report that NFAT5, previously characterized as an osmostress responsive factor, regulates the expression of multiple TLR-induced genes in macrophages independently of osmotic stress. NFAT5 was essential for the induction of the key antimicrobial gene Nos2 (inducible nitric oxide synthase [iNOS]) in response to low and high doses of TLR agonists but is required for Tnf ...[more]

PMID: 22312110

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