Unknown,Transcriptomics,Genomics,Proteomics

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Positive emotion-specific changes in gene expression profile in brain and peripheral tissues of the tickled rats

ABSTRACT: The changes of mRNA expression levels were analyzed in striatum, salivary glands , and peripheral leukocyte of the juvenile rat stimulated by tickling for 4 weeks as a positive stimulation. Keywords: Stress response Twenty-one-day-old male Wistar ratsM-cM-^@M-^@after weaning were used. All rats were separately maintained in the standard cages which were shielded with screens not to see each other during the study. The rats were then subjected to the following three conditions; tickling, light touch, and no stimulus. The stimulation was given to the rats once every weekday (Monday to Friday) for 4 weeks. Tickling stimulation was used as a mimic of the rough-and-tumble play of rats which induced a positive emotional response [50 kHz ultrasonic vocalization (USV)]. Light touch was a discriminative stimulation to tickling, and neither the rat with light touch nor with no stimulus showed such a response in general. Gene expression changes in striatum, salivary glands, and peripheral leukocyte of the tickled rats were comprehensively analyzed comparing with light touched and no stimulated rats using microarray techniques. Biological replicates: 4 animals each condition. One replicate per array.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Katsuyuki Hayashi

PROVIDER: E-GEOD-26354 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Sub-thalamic deep brain stimulation (DBS) reversibly modulates ParkinsonM-bM-^@M-^Ys disease (PD) motor symptoms, providing an unusual opportunity to compare leukocyte transcripts in the same subjects before and after neurosurgery and after disconnecting the stimulus (ON-and OFF-stimulus). Here, we report rapid stimulus-induced and largely reversible changes in PD leukocyte transcripts, which were larger in scope than the disease-induced changes. These transcript changes classified advanced pre- from post-surgery PD patients and discriminated patients from controls. Moreover, the extent of changes correlated with the neurological efficacy of the DBS neurosurgery, and covered both regulatory pathways and individual transcript changes, e.g. SNCA, PARK7 and the splicing factor SFRS1. Following 1 hour OFF-stimulus, these changes were largely reversed. We extracted from these differences a modified transcripts signature which discriminated controls from advanced PD patients, pre- from post-surgery and ON-from OFF-stimulus conditions. A further gene-list independent analysis detected reversed pathways. Our findings suggest future uses of this approach and the discovered molecular signature for early diagnostics of PD and for identifying novel targets for therapeutic intervention in this and other DBS-treatable neurological diseases. 27 Total samples were analyzed. Study design included four conditions. PD patients (n=7) leukocyte blood samples were examined at 3 time points, and healthy control subjects (n=6) were examined once each. Samples were taken 1 day prior to sub-thalamic nucleous (STN) DBS treatment, several months after STN-DBS treatment upon optimal stimulation and following one hour electrical stimulation cessation. The off stimulation caused recruitment of the disease symptoms as measured by the Unified Parkinson's Disease Rating Scale motor section (UPDRS-III). The different stages are designated: S1 (pre-treatment), S2 (post STN-DBS) and S3 (upon off stimulation). All patients were on dopamine replacement therapy (DRT) when examined pre- and post-DBS off stimulation (albeit with reduced therapy dose post-DBS). To reduce biological variability among the samples which is not related to the study, only male subjects were included in this study. Samples from six age- and gender- matched healthy control subjects served to detect disease modified transcripts and for pre- and post- treatment comparisons. The study was approved by the human review board at the Hadassah University Hospital, Ein-Kerem (no. 6-07.09.07) in accordance with the Declaration of Helsinki. All study participants signed informed consent.

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Positive emotion-specific changes in gene expression profile in brain and peripheral tissues of the tickled rats

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