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Transcription profiling of rat dorsal root ganglia from CCI and Seltzer models of neuropathic pain

ABSTRACT: L5 DRG samples from CCI, Seltzer and sham models collected at 14, 21 and 50 days after surgery from ipsilateral hind limb. Note that all replicates are techincal, i.e., there were no biological replicates in this study as samples were pooled for each group

ORGANISM(S): Rattus norvegicus

SUBMITTER: Jakir Ullah

PROVIDER: E-GEOD-2636 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Role of the cysteine protease cathepsin S in neuropathic hyperalgesia.

Barclay Jane J Clark Anna K AK Ganju Pam P Gentry Clive C Patel Sadhana S Wotherspoon Glen G Buxton Frank F Song Chuanzheng C Ullah Jakir J Winter Janet J Fox Alyson A Bevan Stuart S Malcangio Marzia M

Pain 20070123 3

Using a gene expression analysis approach we found that the mRNA encoding the lysosomal cysteine protease cathepsin S (CatS) was up-regulated in rat dorsal root ganglia (DRG) following peripheral nerve injury. CatS protein was expressed in infiltrating macrophages in DRG and near the site of injury. At both sites CatS expression progressively increased from day 3 to day 14 after injury. In naïve rats, intraplantar injection of activated rat recombinant (rr) CatS (0.3, 1 microg/rat) induced a mec ...[more]

PMID: 17250968

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DRG from CCI and Seltzer models of neuropathic pain and sham operated animals

Project description:L5 DRG samples from CCI, Seltzer and sham models collected at 14, 21 and 50 days after surgery from ipsilateral hind limb. Note that all replicates are techincal, i.e., there were no biological replicates in this study as samples were pooled for each group Keywords: ordered

2005-05-21 | GSE2636 | GEO

mRNA expression profiling in the spinal nerve ligation model of neuropathic pain in rats

Project description:Expression profiling of L4 and L5 Dorsal Root Ganglion (DRG) in the spinal nerve ligation model of neuropathic pain. The goal of the study was to identify genes involved in neuropathic pain This series of samples comprises of contralateral and ipsilateral L4 and L5 DRG tissue collected 4 weeks after rats underwent a L5 spinal nerve ligation (SNL) or a sham operation with no L5 spinal nerve ligation. This defines 8 groups (i) contralateral L4 DRG from the sham cohort (n=5), (ii) ipsilateral L4 DRG from sham cohort (n=5), (iii) contralateral L4 DRG from SNL cohort (n=5), (iv) ipsilateral L4 DRG from the SNL chort (n=5), (v) contralateral L5 DRG from the sham cohort (n=5), (vi) ipsilateral L5 DRG from sham cohort (n=5), (vii) contralateral L5 DRG from SNL cohort (n=5), (viii) ipsilateral L5 DRG from the SNL cohort (n=5)

2011-10-17 | E-GEOD-24982 | biostudies-arrayexpress

Transcription profiling of rat dorsal root ganglia from L5 spinal nerve ligation model of neuropathic pain

Project description:DRG samples were extracted at 28 and 50 days from L5 spinal nerve ligated rats and sham operated rats. Samples were pooled for each time point per group and hybridised to duplicate to RG_u34 genchips (A, B and C chips). Experiment Overall Design: Samples were pooled and therefore no biological replication was possible on the arrays

2007-11-06 | E-GEOD-2884 | biostudies-arrayexpress

Transcription profiling of dorsal root ganglia from rats with and without traumatic neuropathic pain from sciatic nerve transection to identify genes responsible for mechanical hypersensitivity

Project description:Comparison of L5 DRG gene expression profiles at day 14 from SNT treated animals vs. sham controls. This experiment is part of larger study, where the expression profiles of three disparate models of neuropathic pain (SNT, VZV infection and gp120+ddC) are compared in order to find genes that are responsible for mechanical hypersensitivity

2008-07-14 | E-MEXP-976 | biostudies-arrayexpress

Integrative multiomic analyses of dorsal root ganglia in diabetic neuropathic pain using proteomics, phosphoproteomics, and metabolomics

Project description:Diabetic peripheral neuropathy (DPN) is characterized by spontaneous pain in the extremities. Incidence of DPN continues to rise with the global diabetes epidemic. However, there remains a lack of safe, effective analgesics to control this chronic painful condition. Dorsal root ganglia (DRG) contain soma of sensory neurons and modulate sensory signal transduction into the central nervous system. In this study, we aimed to gain a deeper understanding of changes in molecular pathways in the DRG of DPN patients with chronic pain. We recently reported transcriptomic changes in the DRG with DPN. Here, we expand upon those results with integrated metabolomic, proteomic, and phosphoproteomic analyses to compare the molecular profiles of DRG from DPN donors and DRG from control donors without diabetes or chronic pain. Our analyses identified decreases of select amino acids and phospholipid metabolites in the DRG from DPN donors, which are important for cellular maintenance. Additionally, our analyses revealed changes suggestive of extracellular matrix (ECM) remodeling and altered mRNA processing. These results reveal new insights into changes in the molecular profiles associated with DPN.

2023-01-09 | MSV000091027 | MassIVE

Whole genome transcription profiling of the L5 spinal nerve transection model of neuropathic pain in the rat, at different sequencing depths (RNA-Seq)

Project description:Poly(A) enriched RNA derived from the L5 DRG 7 days following L5-SNT and from naïve L5-DRG tissue was subjected to RNA-seq analysis at different sequencing depths

2014-01-07 | GSE53762 | GEO

Whole genome transcription profiling of the L5 spinal nerve transection model of neuropathic pain in the rat, at different sequencing depths (Affymetrix transcript-level)

Project description:RNA derived from the L5 DRG 7 days following L5-SNT and from naïve L5-DRG tissue was subjected to microarray analysis and expression was determined at the exon and gene level 6 biological replicates (3 case – SNT subjected L5-DRG tissue, 3 control – naïve L5-DRG tissue). Each sample was made from pooling 4 distinct animals. Sample preparation and hybridization were performed by UCL genomics, following Affymetrix instructions. The 6 replicates were also used for RNA-seq, see GSE53762.

2014-01-07 | E-GEOD-53764 | biostudies-arrayexpress

Whole genome transcription profiling of the L5 spinal nerve transection model of neuropathic pain in the rat, at different sequencing depths (Affymetrix exon-level)

2014-01-07 | E-GEOD-53860 | biostudies-arrayexpress

Expression data from pain model: rat L5 sensory ganglia after local inflammation

Project description:Inflammation plays a role in neuropathic pain conditions as well as in pain induced solely by an inflammatory stimulus. Robust mechanical hyperalgesia and allodynia can be induced by locally inflaming the L5 dorsal root ganglion (DRG) in rat. This model allows investigation of the contribution of inflammation per se to chronic pain conditions. Most previous microarray studies of DRG gene expression have investigated neuropathic pain models involving axon transection. To examine the role of inflammation, we used microarray methods to examine gene expression 3 days after local inflammation of the L5 DRG in rat. We observed significant regulation in a large number of genes (23% of observed transcripts), and examined 221 (3%) with a fold-change of 1.5-fold or more in more detail. Immune-related genes were the largest category in this group and included members of the complement system as well as several pro-inflammatory cytokines. However, these upregulated cytokines had no prior links to peripheral pain in the literature other than through microarray studies, though most had previously described roles in CNS (especially neuroinflammatory conditions) as well as in immune responses. The L5 dorsal root ganglion (DRG) was locally inflamed with zymosan/Incomplete Freund's Adjuvant. DRG were isolated 3 days later. Each sample was RNA extracted from a single DRG. 6 samples from rats with local DRG inflammation were compared with 6 samples from sham-operated rats.

2012-06-21 | E-GEOD-38859 | biostudies-arrayexpress

Postoperative pain and dorsal root ganglia transcriptomic signatures in a novel rat model of total knee arthroplasty

Project description:These data are from a preclinical model of total knee arthroplasty (TKA) performed in healthy rats. One day after surgery, ipsilateral dorsal root ganglia (DRG) from L3 and L4 were collected for RNA extraction and sequencing.

2022-03-23 | GSE195833 | GEO

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