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Expression data in UPEC cystitis in female C57BL/6 mice

ABSTRACT: Data defines for the first time a whole bladder transcriptome of UPEC cystitis in female C57BL/6 mice using genome-wide expression profiling to map early host response pathways stemming from UPEC colonization We used microarrays to detail the global programme of gene expression in UPEC UTI in mice Whole mouse bladder collected at 2h following transurethral infection for RNA extraction and hybridization on Affymetrix microarrays. Non-pooled samples were used, with one array per mouse to expand power of expression profiles. Control mice received only PBS as a mock infection. Group sizes were five mice per group, and therefore five arrays were used per group.

ORGANISM(S): Mus musculus

SUBMITTER: Glen Ulett

PROVIDER: E-GEOD-26509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Innate transcriptional networks activated in bladder in response to uropathogenic Escherichia coli drive diverse biological pathways and rapid synthesis of IL-10 for defense against bacterial urinary tract infection.

Duell Benjamin L BL Carey Alison J AJ Tan Chee K CK Cui Xiangqin X Webb Richard I RI Totsika Makrina M Schembri Mark A MA Derrington Petra P Irving-Rodgers Helen H Brooks Andrew J AJ Cripps Allan W AW Crowley Michael M Ulett Glen C GC

Journal of immunology (Baltimore, Md. : 1950) 20111219 2

Early transcriptional activation events that occur in bladder immediately following bacterial urinary tract infection (UTI) are not well defined. In this study, we describe the whole bladder transcriptome of uropathogenic Escherichia coli (UPEC) cystitis in mice using genome-wide expression profiling to define the transcriptome of innate immune activation stemming from UPEC colonization of the bladder. Bladder RNA from female C57BL/6 mice, analyzed using 1.0 ST-Affymetrix microarrays, revealed e ...[more]

PMID: 22184725

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