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Upregulation of pro-apoptotic microRNA mir-125a following massive small bowel resection in rats

ABSTRACT: Upregulation of mir-125a suppresses the pro-survival protein Mcl1, producing the increase in apoptosis known to accompany the proliferative changes characteristic of intestinal adaptation. Our data highlight a potential role for microRNAs as mediators of the adaptive process and may facilitate the development of new therapeutic options for short bowel syndrome. Two-condition experiment: resected rat jejunum vs. pooled transected control, with dye-swaps.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Jonathan Dreyfuss

PROVIDER: E-GEOD-26542 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Upregulation of proapoptotic microRNA mir-125a after massive small bowel resection in rats.

Balakrishnan Anita A Stearns Adam T AT Park Peter J PJ Dreyfuss Jonathan M JM Ashley Stanley W SW Rhoads David B DB Tavakkolizadeh Ali A

Annals of surgery 20120401 4

<h4>Objective</h4>Short bowel syndrome remains a condition of high morbidity and mortality, and current therapeutic options carry significant side effects. To identify new treatments we focused on postresection changes in microRNAs--short noncoding RNAs, which suppress target genes--and suggest a previously undiscovered role for microRNA-125a (mir-125a) in intestinal adaptation.<h4>Methods</h4>Rats underwent either 80% massive small bowel resection or transection and were harvested after 48 hour ...[more]

PMID: 22418008

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