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Human Induced Pluripotent Stem Cells Harbor Homoplasmic and Heteroplasmic Mitochondrial DNA Mutations While Maintaining human embryonic stem cells-like Metabolic Reprogramming

ABSTRACT: Gene expression analyis of two hESCs, two human neonatal fibroblasts, and four human iPSCs generated with retroviral transduction using the OSKM cocktail. Total mRNA obtained from two fibroblasts (BJ and HFF1), two hESCs (H1 and H9), two BJ-derived iPSCs (iB4 and iB5), and two HFF1-derived iPSCs (iPS2 and iPS4).

ORGANISM(S): Homo sapiens

SUBMITTER: Alessandro Prigione

PROVIDER: E-GEOD-26575 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human induced pluripotent stem cells harbor homoplasmic and heteroplasmic mitochondrial DNA mutations while maintaining human embryonic stem cell-like metabolic reprogramming.

Prigione Alessandro A Lichtner Björn B Kuhl Heiner H Struys Eduard A EA Wamelink Mirjam M Lehrach Hans H Ralser Markus M Timmermann Bernd B Adjaye James J

Stem cells (Dayton, Ohio) 20110901 9

Human induced pluripotent stem cells (iPSCs) have been recently found to harbor genomic alterations. However, the integrity of mitochondrial DNA (mtDNA) within reprogrammed cells has yet to be investigated. mtDNA mutations occur at a high rate and contribute to the pathology of a number of human disorders. Furthermore, the lack of mtDNA integrity may alter cellular bioenergetics and limit efficient differentiation. We demonstrated previously that the derivation of iPSCs is associated with mitoch ...[more]

PMID: 21732474

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