Unknown,Transcriptomics,Genomics,Proteomics

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Expression data of alloantigen stimulated splenocytes treated with leukocyte costimulatory blockade antibodies or no treatment


ABSTRACT: To elucidate the gene expression “footprint” of antigenically challenged T-cells which had been treated with anti-LFA-1, CTLA4Ig, anti-CD40-ligand antibodies, we performed microarray gene expression analysis comparing the expression profile of costimulatory blockade treated and untreated responder T-cells. Allogeneic murine splenocytes were co-cultured in an vitro set-up for a mixed lymphocyte reaction. For the experimental/treated group, the leuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligand were added to the media, the control group did not have any antiodies added to the media. After 5 days of incubation the responder CD4 and CD8 T cells were isolated by FACS and the gene expression was analyzed using affymetrix microarray platforms.

ORGANISM(S): Mus musculus

SUBMITTER: Kitchener Wilson 

PROVIDER: E-GEOD-26669 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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