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Zebrafish embryos: hdac1 Morphants vs Standard control morphants

ABSTRACT: Transcriptional profiling of hdac1 morphant zebrafish embryos in comparison to standard control injected embryos. Embryos where injected at the one cell stage with either a translation blocking morpholino (Hdac1 ATG), splice blocking morpholino (Hdac1 Splice) or Hdac1 mismatch control morpholino (Hdac1 control). RNA was extracted and compared in a two-colour hybridisation to the comman reference Standard control morpholino injected embryos. Common reference experiment, hdac1 morphants vs. standard control morphants. Biological replicates: 2 (except Splice which was technical rather than biological)

ORGANISM(S): Danio rerio

SUBMITTER: Michael Harrison

PROVIDER: E-GEOD-26708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The epigenetic regulator Histone Deacetylase 1 promotes transcription of a core neurogenic programme in zebrafish embryos.

Harrison Michael R M MR Georgiou Aristophanes S AS Spaink Herman P HP Cunliffe Vincent T VT

BMC genomics 20110112

<h4>Background</h4>The epigenetic regulator Histone Deacetylase 1 (Hdac1) is required for specification and patterning of neurones and myelinating glia during development of the vertebrate central nervous system (CNS). This co-ordinating function for Hdac1 is evolutionarily conserved in zebrafish and mouse, but the mechanism of action of Hdac1 in the developing CNS is not well-understood.<h4>Results</h4>A genome-wide comparative analysis of the transcriptomes of Hdac1-deficient and wild-type zeb ...[more]

PMID: 21226904

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Zebrafish embryos: hdac1 Morphants vs Standard control morphants

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