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Zebrafish embryos: hdac1 Morphants vs Standard control morphants

ABSTRACT: Transcriptional profiling of hdac1 morphant zebrafish embryos in comparison to standard control injected embryos. Embryos where injected at the one cell stage with either a translation blocking morpholino (Hdac1 ATG), splice blocking morpholino (Hdac1 Splice) or Hdac1 mismatch control morpholino (Hdac1 control). RNA was extracted and compared in a two-colour hybridisation to the comman reference Standard control morpholino injected embryos. Common reference experiment, hdac1 morphants vs. standard control morphants. Biological replicates: 2 (except Splice which was technical rather than biological)

ORGANISM(S): Danio rerio

SUBMITTER: Michael Harrison

PROVIDER: E-GEOD-26708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The epigenetic regulator Histone Deacetylase 1 promotes transcription of a core neurogenic programme in zebrafish embryos.

Harrison Michael R M MR Georgiou Aristophanes S AS Spaink Herman P HP Cunliffe Vincent T VT

BMC genomics 20110112

<h4>Background</h4>The epigenetic regulator Histone Deacetylase 1 (Hdac1) is required for specification and patterning of neurones and myelinating glia during development of the vertebrate central nervous system (CNS). This co-ordinating function for Hdac1 is evolutionarily conserved in zebrafish and mouse, but the mechanism of action of Hdac1 in the developing CNS is not well-understood.<h4>Results</h4>A genome-wide comparative analysis of the transcriptomes of Hdac1-deficient and wild-type zeb ...[more]

PMID: 21226904

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Zebrafish embryos: hdac1 Morphants vs Standard control morphants

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2011-01-21 | GSE26708 | GEO

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2011-01-21 | E-GEOD-26709 | biostudies-arrayexpress

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Project description:We have characterised the zebrafish ortholog, setb, and investigated its role in embryogenesis. Phylogenetic analysis showed that zebrafish Setb has an amino acid sequence identity of approximately 96% with the mammalian orthologs. Whole mount immunofluorescence analysis revealed that Setb is expressed mainly in the eye, the lateral line neuromasts and the olfactory pit. Knockdown of setb using antisense morpholino oligonucleotides resulted in increased apoptosis, reduced cell proliferation and severe morphological defects. The morphant phenotypes were partially rescued when setb MO1 was co-injected with human set mRNA. In vivo labelling of hair cells in the lateral line of setb morphants with the vital fluorescent dye FM1-43 showed a significant decreased number of functional neuromasts. Gene expression analysis of setb morphants, employing DNA microarrays revealed a role of Setb in neurogenesis and the mechanosensory lateral line system. 48hpf control morpholino- and setb MO1-injected embryos in triplicates

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