Unknown,Transcriptomics,Genomics,Proteomics

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Mast cell microRNAs regulated by KIT signaling


ABSTRACT: Objective is to identify microRNAs regulated by KIT signaling in mast cells. miRNA profiles of murine bone marrow derived mast cells with and without SCF treatment were compared. In addition, miRNA profiles of the P815 mutant KIT cell line with and without the KIT inhibitor, imatinib, were compared. We identified miRNAs that were differentially regulated by KIT signals. Two systems were used to identify microRNAs regulated by KIT. RNA was collected from primary murine bone marrow derived mast cells (BMMC) treated and not treated with KIT ligand (SCF). In addition, RNA was harvested from the P815 (activating KIT mutation positive) cell line treated and not treated with the KIT inhibitor, imatinib. Samples were harvested after 24 hours of treatment

ORGANISM(S): Mus musculus

SUBMITTER: Cliff Takemoto 

PROVIDER: E-GEOD-26783 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

KIT signaling regulates MITF expression through miRNAs in normal and malignant mast cell proliferation.

Lee Youl-Nam YN   Brandal Stephanie S   Noel Pierre P   Wentzel Erik E   Mendell Joshua T JT   McDevitt Michael A MA   Kapur Reuben R   Carter Melody M   Metcalfe Dean D DD   Takemoto Clifford M CM  

Blood 20110127 13


Activating mutations in codon D816 of the tyrosine kinase receptor, KIT, are found in the majority of patients with systemic mastocytosis. We found that the transcription factor, microphthalmia-associated transcription factor (MITF), is highly expressed in bone marrow biopsies from 9 of 10 patients with systemic mastocytosis and activating c-KIT mutations. In primary and transformed mast cells, we show that KIT signaling markedly up-regulates MITF protein. We demonstrate that MITF is required fo  ...[more]

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