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SMAD3 targets in A549 lung epithelial cells

ABSTRACT: TGF? activates a signal transduction cascade that results in the transcription of genes, primarily through the DNA-binding transcription factor SMAD3. The objective of this study is to identify SMAD3 binding targets and the molecular pathways affected by the TGF?1/SMAD3 signal transduction on a genome-wide scale. Cultured A549 cells at 30-50% confluence were treated with 10 uM SIS3 in dimethyl sulfoxide (DMSO), or DMSO (vehicle-only) 30 min prior to TGF?1 treatment. Cells were treated with 2 ng/mL recombinant TGF?1 for 0, 2, 12, and 24 h.

ORGANISM(S): Homo sapiens

SUBMITTER: Kusum Pandit

PROVIDER: E-GEOD-26858 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High throughput determination of TGFβ1/SMAD3 targets in A549 lung epithelial cells.

Zhang Yingze Y Handley Daniel D Kaplan Tommy T Yu Haiying H Bais Abha S AS Richards Thomas T Pandit Kusum V KV Zeng Qilu Q Benos Panayiotis V PV Friedman Nir N Eickelberg Oliver O Kaminski Naftali N

PloS one 20110520 5

<h4>Background</h4>Transforming growth factor beta 1 (TGFβ1) plays a major role in many lung diseases including lung cancer, pulmonary hypertension, and pulmonary fibrosis. TGFβ1 activates a signal transduction cascade that results in the transcriptional regulation of genes in the nucleus, primarily through the DNA-binding transcription factor SMAD3. The objective of this study is to identify genome-wide scale map of SMAD3 binding targets and the molecular pathways and networks affected by the T ...[more]

PMID: 21625455

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