Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum
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ABSTRACT: Comparison of the gene expression profile of Moraxella catarrhalis grown in the presence of 20% pooled human sputum in chemically-defined medium relative to Moraxella catarrhalis grown in chemically-defined medium alone. Moraxella catarrhalis ATCC43617 was grown to mid-logarithmic phase either in the presence of 20% pooled human sputum in chemically-defined medium or in chemically-defined medium alone. Total RNA was extracted from bacterial cells exposed to each of these conditions and cDNA was generated for CyDye labelling. 3 biologic replicates were generated and each replicate underwent a dye swap (total of 6 experimental data collections). The gene expression profile reported is that of Moraxella catarrhalis grown in the presence of pooled human sputum in a chemically-defined medium relative to Moraxella catarrhalis grown only in the presence of the chemically-defined medium.
ORGANISM(S): Moraxella catarrhalis ATCC 43617
SUBMITTER: Todd Hoopman
PROVIDER: E-GEOD-27059 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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