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Expression data from self-deprived regulatory CD4 T cells

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE26714: Expression data from Tregs adoptively transferred in MHC II competent or deficient recipients GSE27151: Expression data from Tregs purified from WT-CD3KO or IIKO-CD3KO chimeras Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: florent dumont

PROVIDER: E-GEOD-27153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from self-deprived regulatory CD4 T cells

Project description:Disruption of TCR /MHC class II interactions leads rapidly to alterations of the common CD4 Treg transcriptional signature Self-deprived, non-functional Tregs were compare to fully functional Tregs by microarrays. Total T cells from the periphery of WT mice were adoptively transferred into CD3ε-/- recipient mice lacking or not MHC class II molecule expression (MHC II- or MHC II+ recipient mice, respectively). Five days later, peripheral Tregs transferred in MHC II - competent (CD4CD25B6) or - deficient (CD4CD25IIko) recipient were purified for RNA extraction and hybridization on Affymetrix microarrays.

2011-01-20 | E-GEOD-26714 | biostudies-arrayexpress

Expression data from Tregs purified from WT-CD3KO or IIKO-CD3KO chimeras

Project description:Disruption of TCR /MHC class II interactions leads rapidly to alterations of the common CD4 Treg transcriptional signature Self-deprived, non-functional Tregs were compare to fully functional Tregs by microarrays. CD3ε-/- mice were lethally irradiated and their immune system reconstituted with WT mouse (WT-CD3KO chimeras) or IIKO mouse (IIKO-CD3KO) Bone Marrow cells. Twenty eight days later, peripheral Tregs from these chimeras were purified for RNA extraction and hybridization on Affymetrix microarrays.

2011-02-09 | E-GEOD-27151 | biostudies-arrayexpress

TIME-COURSE GENE EXPRESSION OF Metharizium anisopliae GROWING IN PLANT ROOT EXUDATE

Project description:The presence of genetic groups of the entomopathogenic fungus Metarhizium anisopliae in soil is shaped by its adaptability to specific soil and habitat types, and by soil insect populations. Although the entomopathogenic life style of this fungus is well studied, its saprophytic life style has received little consideration. While a set of functionally related genes can be commonly expressed for the adaptability of this fungus to different environments (insect cuticle, insect blood and root exudates), a different subset of genes is also expected for each environment. In order to increase the knowledge of the potential use of M. anisopliae as a rhizosphere competent organism, in this study we evaluated the genetic expression of this fungus while growing on plant root exudates in laboratory conditions during a time course. One fungal strain: Metarhizium anisopliae ARSEF 2575; Five time conditions: 0h, 1h, 4h, 8h, 12h; Five-condition experiment: Time0h vs. Time1h, Time1h vs. Time4h, Time4h vs. Time8h, Time8h vs. Time12h and Time12h vs. Time0h. Two Biological replicates: independently grown and harvested. Three replicates per array. Dye-swap was performed on replicate 2.

2010-12-29 | E-GEOD-16848 | biostudies-arrayexpress

Mus musculus

Project description:Expression data from Tregs adoptively transferred in MHC II competent or deficient recipients

| PRJNA142147 | ENA

Towards understanding the biological function of the unusual chaperonin GroEL1 of Mycobacterium tuberculosis

Project description:Expression data from wild type (H37Rv) and GroEL1(H37RvΔgroEL1::Hyg) mutant strains under a range of environmental stresses The 60 kDa heat shock proteins, also known as GroELs are components of the essential protein folding machinery of the cell, but are also dominant antigens in many infectious diseases. Although generally essential for cellular survival, in some organisms such as Mycobacterium tuberculosis, one or more paralogous GroELs are known to be dispensable. In M. tuberculosis, groEL2 is essential for cell survival, but the biological role of the non-essential GroEL1 is still elusive. To understand the relevance of GroEL1 in M. tuberculosis physiology, detailed transcriptomic analyses for the wild type H37Rv and groEL1 knockout (groEL1 KO) were performed under in vitro stress conditions: stationary phase, cold shock, low aeration, mild cold shock and low pH. Additionally, the survival of the groEL1 KO was assessed in macrophages at multiplicity of infection (MOI) of 1:1 and 1:5. We observed that survival under low aeration was significantly compromised in the groEL1 KO. Further, the gene expression analyses under low aeration showed change in expression of several key virulence factors like two component system PhoP/R and MprA/B, sigma factors sigG, M and H and adversely affected known hypoxia response regulators Rv0081, Rv0023 and DosR. Our work is therefore suggestive of an important role of GroEL1 for survival under low aeration by affecting the expression of genes known for hypoxia response. Expression data from wild type and GroEL1 mutant strains in response to 8 environmental stresses plus log phase growth, with at least three biological replicates of each.

2015-05-14 | E-GEOD-68856 | biostudies-arrayexpress

Transcription profiling of mouse HA specific T regulatory cells cultured in the presence of HA-pulsed dendritic cells and rhIL-2 with or without HA-specific T effector cells

Project description:Expanded HA-specific Thy-1.1 Tregs were cultured in the presence of HA-pulsed dendritic cells and rhIL-2 with or without HA-specific Teffs. After 5 days, Tregs were sorted by flow cytometry, based on the expression of the Thy-1.1 congeneic marker. Two biological duplicates were hybridized to the Sentrix BeadChips Array mouse WG-6 v2 (Illumina, USA). Data extraction and normalization was performed using Quantile normalization in the BeadStudio software.

2009-09-08 | E-MTAB-146 | biostudies-arrayexpress

Transcription profiling of Aspergillus fumigatus hyphae and conidia after incubation with neutrophils from different donors

Project description:Hyphae and conidia, representing the two developmental stages of the fungus, were examined separately in this study. RNA from hyphae or conidia incubated in plasma in RPMI/HEPES without neutrophils was used as reference, and was co-hybridized with the query samples obtained from the corresponding sets incubated with neutrophils. Each biological replicate was carried out with neutrophils obtained from a different donor, and the RNA samples associated with each neutrophil source was paired separately with a corresponding reference sample prepared at the same time.

2008-09-25 | E-MEXP-1722 | biostudies-arrayexpress

Bovine granulosa cells of dominant follicles; comparison of growing vs plateau

Project description:Granulosa cells originating from two different phases of antral follicle growth (growing and plateau) were compared in follicles with a diameter of 9mm or more. Growing follicle is the reference. Two conditions experiment (Growing and Plateau); Four biological replicates for each group; Two technical replicates per samples (dye-swap).

2015-03-06 | E-GEOD-63904 | biostudies-arrayexpress

Bovine granulosa cells of dominant follicles; comparison of growing vs atretic

Project description:Granulosa cells originating from two different phases of antral follicle growth (growing and atresia) were compared in follicles with a diameter of 9mm or more. Growing follicle is the reference. Two conditions experiment (Growing and Atresia); Four biological replicates for each group; Two technical replicates per samples (dye-swap).

2015-03-06 | E-GEOD-63918 | biostudies-arrayexpress

Bovine granulosa cells of dominant follicles; comparison of plateau vs atretic

Project description:Granulosa cells originating from two different phases of antral follicle growth (plateau and atretic) were compared in follicles with a diameter of 9mm or more. The plateau follicle is the reference. Two conditions experiment (Plateau and Atretic); Four biological replicates for each group; Two technical replicates per samples (dye-swap).

2015-03-06 | E-GEOD-63919 | biostudies-arrayexpress

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