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Cardiac gene expression profiling of Salvador mutant mice

ABSTRACT: In this study, we identified a number of genes whose expression are regulated by the Hippo tumor suppressor pathway. To disrupt Hippo signaling in the mouse heart, we inactivated the single mammalian Salv ortholog using a Salv conditional null allele and the Nkx2.5 cre allele that directs cardiac cre activity. Total RNA from embryonic hearts were used for expression profiling of 17,455 unique genes. Genes transcriptionally regulated by the Hippo pathway during cardiogenesis were identifed through expression profiling of 17,455 unique genes in Salvador mutant (Salv CKO) embryonic hearts. Total RNA from E9.5-stage hearts were pooled into biological replicate samples (2 control and 2 Salv CKO) and were used to generate expression profiles.

ORGANISM(S): Mus musculus

SUBMITTER: Todd Heallen

PROVIDER: E-GEOD-27259 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hippo pathway inhibits Wnt signaling to restrain cardiomyocyte proliferation and heart size.

Heallen Todd T Zhang Min M Wang Jun J Bonilla-Claudio Margarita M Klysik Ela E Johnson Randy L RL Martin James F JF

Science (New York, N.Y.) 20110401 6028

Genetic regulation of mammalian heart size is poorly understood. Hippo signaling represents an organ-size control pathway in Drosophila, where it also inhibits cell proliferation and promotes apoptosis in imaginal discs. To determine whether Hippo signaling controls mammalian heart size, we inactivated Hippo pathway components in the developing mouse heart. Hippo-deficient embryos had overgrown hearts with elevated cardiomyocyte proliferation. Gene expression profiling and chromatin immunoprecip ...[more]

PMID: 21512031

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