Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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MMP101


ABSTRACT: Methanococcus maripaludis is a methanogenic archaeon. Within its genome, there are two operons for membrane associated hydrogenases, eha and ehb. To investigate the regulation of ehb on the cell, an S40 mutant was constructed in such a way that a portion of the ehb operon was replaced by pac cassette in the wild type parental strain S2 (done by Whitman's group at the University of Georgia). Four samples of each strain were grown in batch culture. Differences in transcriptional expression between S40 and S2 were measured using cDNA arrays, with flip dye experiments for each biological replicate. 16 replicates were used for the experiment. Four biological replicates of the wild type (S2) and mutant (S40) strains where compared using dye flips. Each slide contained two copies of the array yielding four technical replicates for each biological replicate.

ORGANISM(S): Methanococcus maripaludis

SUBMITTER: John Leigh 

PROVIDER: E-GEOD-2745 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Quantitative proteomics of the archaeon Methanococcus maripaludis validated by microarray analysis and real time PCR.

Xia Qiangwei Q   Hendrickson Erik L EL   Zhang Yi Y   Wang Tiansong T   Taub Fred F   Moore Brian C BC   Porat Iris I   Whitman William B WB   Hackett Murray M   Leigh John A JA  

Molecular & cellular proteomics : MCP 20060217 5


For the archaeon Methanococcus maripaludis, a fully sequenced and annotated model species of hydrogenotrophic methanogen, we report validation of quantitative protein level expression ratios on a proteome-wide basis. Using an approach based on quantitative multidimensional capillary HPLC and quadrupole ion trap mass spectrometry, coverage of gene expression approached that currently achievable with transcription microarrays. Comprehensive mass spectrometry-based proteomics and spotted cDNA array  ...[more]

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