Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

SCREENING STUDY TO IDENTIFY DIAGNOSTIC MARKERS FOR LUNG CANCER IN ENDOBRONCHIAL LINING FLUID

ABSTRACT: We investigated whether biomarker analysis in endobronchial epithelial lining fluid (ELF) collected by bronchoscopic microsampling may be useful for a definitive preoperative diagnosis. Therefore we compared ELF samples close to nodule and from the contralateral site from patients with malignant or benign diagnosis. ELF samples have been derived from early stage NSCLC patients and controls. LIMMA analysis was performed to identify differentially expressed genes associated to a malignant diagnosis. keywords: disease subtype analysis

ORGANISM(S): Homo sapiens

SUBMITTER: Ruprecht Kuner

PROVIDER: E-GEOD-27489 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Early detection of lung cancer by molecular markers in endobronchial epithelial-lining fluid.

Kahn Nicolas N Meister Michael M Eberhardt Ralf R Muley Thomas T Schnabel Philipp A PA Bender Christian C Johannes Marc M Keitel Denise D Sültmann Holger H Herth Felix J F FJ Kuner Ruprecht R

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 20120601 6

<h4>Introduction</h4>Early detection of malignancies in the lung by less-invasive methods aims at achieving efficient intervention and subsequently a reduction of the high mortality rate. We investigated whether biomarker analysis in endobronchial epithelial-lining fluid (ELF) collected by bronchoscopic microsampling (BMS) may be useful for a definitive preoperative diagnosis.<h4>Methods</h4>ELF was collected from subsegmental bronchi close to the indeterminate pulmonary nodule, which was detect ...[more]

PMID: 22588153

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Project description:Maintenance and maturation of primordial germ cells is controlled by complex genetic and epigenetic cascades, and disturbances in this network lead to either infertility or malignant aberration. Transcription factor Tcfap2c / TFAP2C has been described to be essential for primordial germ cell maintenance and to be upregulated in several human germ cell cancers. Using global gene expression profiling, we identified genes deregulated upon loss of Tcfap2c in primordial germ cell-like cells. We show that loss of Tcfap2c affects many aspects of the genetic network regulating germ cell biology, such as downregulation maturation markers and induction of markers indicative of somatic differentiation, cell cycle, epigenetic remodeling, and pluripotency associated genes. Chromatin-immunoprecipitation analyses demonstrated binding of Tcfap2c to regulatory regions of deregulated genes (Sfrp1, Dmrt1, Nanos3, c-Kit, Cdk6, Cdkn1a, Fgf4, Klf4, Dnmt3b and Dnmt3l) suggesting that these genes are direct transcriptional targets of Tcfap2c in primordial germ cells. Since Tcfap2c deficient primordial germ cell like cells display cancer related deregulations in epigenetic remodeling, cell cycle and pluripotency control, the Tcfap2c-knockout allele was bred onto 129S2/Sv genetic background. There, mice heterozygous for Tcfap2c develop germ cell cancer with high incidence. Precursor lesions can be observed as early as E16.5 in developing testes displaying persisting expression of pluripotency markers. We further demonstrate, that mice with a heterozygous deletion of the Tcfap2c target gene Nanos3 are also prone to develop teratoma. These data highlight Tcfap2c as a critical and dose-sensitive regulator of germ cell fate. 8 samples were analyzed. Ctrl ESC: Control mouse embryonic stem cells (ESCs), 2 biological rep KO ESC: Tcfap2c knock-out mouse embryonic stem cells (ESCs), 2 biological rep Ctrl PGC: Control mouse primordial germ cells (PGCs), 2 biological rep KO PGC: Tcfap2c knock-out mouse primordial germ cells (PGCs), 2 biological rep

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Dataset Information

SCREENING STUDY TO IDENTIFY DIAGNOSTIC MARKERS FOR LUNG CANCER IN ENDOBRONCHIAL LINING FLUID

Publications

Early detection of lung cancer by molecular markers in endobronchial epithelial-lining fluid.

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