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Expression data from BIO-treated human epidermal keratinocytes

ABSTRACT: Human keratinocytes isolated from foreskin were cultured and treated with a GSK-3beta inhibitor, BIO. The effect of BIO was evaluated by the cell growth, clony formation and differentiating markers. We used microarrays to detail the global program of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Human primary keratinocytes were subjected for RNA extraction. The total RNAs were hybridized on Affymetrix microarrays. The cultured cells exhibit a homogous cell morphology at passage 1 to passage 5. The cells were treated with BIO at passage 3 or 4.

ORGANISM(S): Homo sapiens

SUBMITTER: Hong-Lin Su

PROVIDER: E-GEOD-27509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from BIO-treated human epidermal keratinocytes

Project description:Human keratinocytes isolated from foreskin were cultured and treated with a GSK-3beta inhibitor, BIO. The effect of BIO was evaluated by the cell growth, clony formation and differentiating markers. We used microarrays to detail the global program of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process.

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Expression data from human primary keratinocytes

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