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Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE27512: Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (miRNA) GSE27513: Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (mRNA) Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Karlheinz Holzmann

PROVIDER: E-GEOD-27514 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The clathrin-binding domain of CALM and the OM-LZ domain of AF10 are sufficient to induce acute myeloid leukemia in mice.

Deshpande A J AJ Rouhi A A Lin Y Y Stadler C C Greif P A PA Arseni N N Opatz S S Quintanilla-Fend L L Holzmann K K Hiddemann W W Döhner K K Döhner H H Xu G G Armstrong S A SA Bohlander S K SK Buske C C

Leukemia 20110617 11

The t(10;11)(p13-14;q14-21) translocation, giving rise to the CALM-AF10 fusion gene, is a recurrent chromosomal rearrangement observed in patients with poor prognosis acute myeloid leukemia (AML). Although splicing of the CALM-AF10 fusion transcripts has been described in AML patients, the contribution of different CALM and AF10 domains to in vivo leukemogenesis remains to be defined. We therefore performed detailed structure-function studies of the CALM-AF10 fusion protein. We demonstrate that ...[more]

PMID: 21681188

Similar Datasets

Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (mRNA)

Project description:The t(10;11) p (13;q14) translocation, giving rise to CALM-AF10, is a recurring chromosomal translocation observed in several types of acute leukemias as well as in lymphoma. We have previously demonstrated that the expression of the human CALM/AF10 fusion gene in murine bone marrow stem and progenitor cells results in an aggressive acute myeloid leukemia in vivo. In this study, we have screened the various domains essential for CALM-AF10 function and leukemogenicity. Our study identifies a mutant of CALM-AF10 that greatly enhances the clonogenic potential of hematopoietic progenitors while retaining key characteristics of disease induced by the full length CALM-AF10 fusion. Global gene expression of bone marrow cells transduced with various constructs were compared. We used the empty vector, MIG, as a control and baseline. Four samples are tested with three biological replicates each.

2011-10-04 | E-GEOD-27513 | biostudies-arrayexpress

Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (miRNA)

Project description:The t(10;11) p (13;q14) translocation, giving rise to CALM-AF10, is a recurring chromosomal translocation observed in several types of acute leukemias as well as in lymphoma. We have previously demonstrated that the expression of the human CALM/AF10 fusion gene in murine bone marrow stem and progenitor cells results in an aggressive acute myeloid leukemia in vivo. In this study, we have screened the various domains essential for CALM-AF10 function and leukemogenicity. Our study identifies a mutant of CALM-AF10 that greatly enhances the clonogenic potential of hematopoietic progenitors while retaining key characteristics of disease induced by the full length CALM-AF10 fusion. Global micro-RNA expression of bone marrow cells transduced with various constructs were compared. We used the empty vector, MIG, as a control and baseline. Four samples are tested with three biological replicates each.

2011-10-04 | E-GEOD-27512 | biostudies-arrayexpress

Project description:Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant

| PRJNA138581 | ENA

Mus musculus

Project description:Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (miRNA)

| PRJNA141867 | ENA

Mus musculus

Project description:Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (mRNA)

| PRJNA141749 | ENA

Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (mRNA)

2011-10-05 | GSE27513 | GEO

Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (miRNA)

2011-10-05 | GSE27512 | GEO

CALM-AF10 T-ALL

Project description:Comparison of a series of 6 CALM-AF10 positive T-ALL to a series of 17 CALM-AF10 negative T-ALL Keywords: ordered

2005-07-01 | GSE2117 | GEO

CRISPR Screening for CALM-AF10 interactome and transcriptional targets

Project description:We performed pooled CRISPR screens to determine the whether the CALM-AF10 target genes and protein interactors identified by RNA-Seq and proteomics methods are functional dependencies for CALM-AF10-driven AML. We constructed a pooled CRISPR library of CALM-AF10 mouse target genes and interactors, and used it to transduce CALM-AF10 mouse AMLs. The cells were sampled at "Day 0" and kept in culture to determine in vitro dependencies at day 12. For in vivo screens, the input cell pool from day 0 was injected in mice and mice where sacrificed upon signs of AML disease, and the AML cells were harvested.

2021-01-05 | GSE164183 | GEO

Gene expression profiling of CA10 and NHD13 murine AML

Project description:We have compared the gene expression profiles of leukemic tissues in two different mouse models of AML, CALM-AF10 and NHD13, to clincally healthy transgenic and wildtype hematopoetic tissue to identify genes and pathways that can collaborate with these oncogenic fusion proteins to promote leukemic transformation. CA10 and NHD13 transgenic mice were sacrificed when clinical signs of leukemia manifested (mice were hunched, skinny, or had an abnormal complete blood count). For comparsion, we harvested age matched bone marrow, thymi, and spleen from wildtype and clinically healthy trangenic tissues. Transgenic animals were considered clinically healthy if no evident signs of leukemia were present.

2012-09-01 | E-GEOD-40531 | biostudies-arrayexpress

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