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MMTV tumor and host cells

ABSTRACT: MMTV Wnt-1 and MMTV-Her2 tumors are transplanted onto GFP transgenic mice. Separate GFP+ cells and GFP- cells by FACS sorting to see the oncogene dependent gene expression changes in both tumor cell compartment (GFP- cells). Wnt Fzd 6hrs group: Wnt inhibitor (Fzd8-CRD) treated tumor RNA which will give some clue for direct Wnt target genes only in tumor cells. GFP- Tumor cells (4 biological replicates) and Wnt-inhibitor treated cells (5 biological replicates)

ORGANISM(S): Mus musculus

SUBMITTER: Suchit Jhunjhunwala

PROVIDER: E-GEOD-27540 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MMTV tumor and host cells

Project description:MMTV Wnt-1 and MMTV-Her2 tumors are transplanted onto GFP transgenic mice. Separate GFP+ cells and GFP- cells by FACS sorting to see the oncogene dependent gene expression changes in both tumor cell compartment (GFP- cells). Wnt Fzd 6hrs group: Wnt inhibitor (Fzd8-CRD) treated tumor RNA which will give some clue for direct Wnt target genes only in tumor cells.

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