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Human monocyte-derived macrophages polarized by GM-CSF or M-CSF

ABSTRACT: Identification of genes differentially expressed between human monocyte-macrophages generated in the presence of either GM-CSF (termed M1) or M-CSF (termed M2) Human peripheral blood monocytes from three independent healthy donors (#1, #2 and #3) were isolated by anti-CD14-labeled magnetic microbeads. CD14+ monocytes were cultured for 7 days in RPMI 10% FCS containing either GM-CSF or M-CSF. Total RNA from each condition was extracted and purified using the RNeasy kit (Qiagen). Labelled RNA was used as hybridization probes on human Codelink Whole genome Bioarray. All experimental procedures were performed following manufacturer instructions. Microarrays were scanned with a GenePix 4000B (Axon Instruments) scanner. Scanned images and raw data were processed using the Codelink Expression Software.

ORGANISM(S): Homo sapiens

SUBMITTER: Angel Corbí

PROVIDER: E-GEOD-27792 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.

Sierra-Filardi Elena E Puig-Kröger Amaya A Blanco Francisco J FJ Nieto Concha C Bragado Rafael R Palomero M Isabel MI Bernabéu Carmelo C Vega Miguel A MA Corbí Angel L AL

Blood 20110309 19

M-CSF favors the generation of folate receptor β-positive (FRβ⁺), IL-10-producing, immunosuppressive, M2-polarized macrophages [M2 (M-CSF)], whereas GM-CSF promotes a proinflammatory, M1-polarized phenotype [M1 (GM-CSF)]. In the present study, we found that activin A was preferentially released by M1 (GM-CSF) macrophages, impaired the acquisition of FRβ and other M2 (M-CSF)-specific markers, down-modulated the LPS-induced release of IL-10, and mediated the tumor cell growth-inhibitory activity o ...[more]

PMID: 21389328

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