DNA methylation analysis of normal and cancer cells

ABSTRACT: DNA methylation of prostatic normal cells (PrEC), normal mammary epithelial cells (HMEC), prostate cancer cell line (MDA-PCa-2b), and breast cancer cell lines (BT474 and MDA-MB-231) was analyzed by MeDIP-on-chip analysis. DNA obtained from each cells was immunoprecipitated by anti 5-methylcytidine antibody. IP DNA and input DNA were labeled with Cy5 and Cy3, respectively, and then analyzed by using human CpG island microarray provided by Agilent Technologies.

ORGANISM(S): Homo sapiens

SUBMITTER: Hideyuki Takeshima 

PROVIDER: E-GEOD-28284 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress