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Skipsey: Fenclorim safening of Arabidopsis


ABSTRACT: Wild type Arabidopsis thaliana Col-0 root cultures, were treated with fenclorim or 4-chloro-6-methyl-2-phenylpyrimidine dissolved in acetone to achieve a final concentration of 100uM. The final acetone concentration of 0.1% was replicated in control root cultures. Samples were taken at four and twenty-four hours post addition in biological triplicate. Root cultures were routinely maintained at 25C in the dark. 18 samples were used in this experiment

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Mark Skipsey 

PROVIDER: E-GEOD-28431 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Xenobiotic responsiveness of Arabidopsis thaliana to a chemical series derived from a herbicide safener.

Skipsey Mark M   Knight Kathryn M KM   Brazier-Hicks Melissa M   Dixon David P DP   Steel Patrick G PG   Edwards Robert R  

The Journal of biological chemistry 20110721 37


Plants respond to synthetic chemicals by eliciting a xenobiotic response (XR) that enhances the expression of detoxifying enzymes such as glutathione transferases (GSTs). In agrochemistry, the ability of safeners to induce an XR is used to increase herbicide detoxification in cereal crops. Based on the responsiveness of the model plant Arabidopsis thaliana to the rice safener fenclorim (4,6-dichloro-2-phenylpyrimidine), a series of related derivatives was prepared and tested for the ability to i  ...[more]

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