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Gene expression analysis in A549 cells

ABSTRACT: Snai1 or ncRNA-a7 were knocked down in A549 cells using siRNA and the changes in mRNA levels were determined. Comparative analysis of duplicate microarray experiments compared to control transfected cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Ulf Ørom

PROVIDER: E-GEOD-28558 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Long noncoding RNAs with enhancer-like function in human cells.

Ørom Ulf Andersson UA Derrien Thomas T Beringer Malte M Gumireddy Kiranmai K Gardini Alessandro A Bussotti Giovanni G Lai Fan F Zytnicki Matthias M Notredame Cedric C Huang Qihong Q Guigo Roderic R Shiekhattar Ramin R

Cell 20101001 1

While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in hum ...[more]

PMID: 20887892

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