Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

FlhC mediated gene regulation In E. coliO157:H7 on meat

ABSTRACT: The study aimed at investigating FlhC mediated gene regulation in E. coli O157:H7 on surface of meat. It also aimed at investigating the phenotypic effect of such a regulation. Two strains WT and FlhC mutant used. FlhC mutant had a 12 base pair deletion. 3 biological replicates . Wt labeled with Alexa 555, FlhC mutant with Alexa 647

ORGANISM(S): Escherichia coli O157:H7

SUBMITTER: Birgit Pruess

PROVIDER: E-GEOD-28836 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Regulation of cell division, biofilm formation, and virulence by FlhC in Escherichia coli O157:H7 grown on meat.

Sule Preeti P Horne Shelley M SM Logue Catherine M CM Prüss Birgit M BM

Applied and environmental microbiology 20110415 11

To understand the continuous problems that Escherichia coli O157:H7 causes as food pathogen, this study assessed global gene regulation in bacteria growing on meat. Since FlhD/FlhC of E. coli K-12 laboratory strains was previously established as a major control point in transducing signals from the environment to several cellular processes, this study compared the expression pattern of an E. coli O157:H7 parent strain to that of its isogenic flhC mutant. This was done with bacteria that had been ...[more]

PMID: 21498760

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Project description:The two-component system (TCS) is a specific regulatory system in bacteria and plays an important role in sensing and adapting to the environment. In this study, we evaluated the roles of TCSs in the major cariogenic pathogen Streptococcus mutans in resistance to several types of bacteriocin. In a comprehensive analysis using individual TCS mutants, we found that two novel TCSs were associated with resistance against distinct lantibiotics nisin A (class I type A[I]) and nukacin ISK-1(class I type A[II]). One TCS, SMU.659-660 (designated as NsrRS), was related to resistance against nisin A produced by Lactococcus lactis ATCC 11454. The other TCS, SMU.1146-1145 (designated as LcrRS), was related to resistance against nukacin ISK-1 produced by Staphylococcus warneri ISK-1. NsrRS induced the expression of SMU.658 (designated as NsrX), which constitutes an operon with nsrRS, in response to nisin A. Inactivation of nsrX increased susceptibility to nisin A. Additionally, NsrX expression in S. mutans increased the binding affinity to nisin A compared to a no-expression strain. LcrRS induced the expression of SMU.1148-50 (lctFEG), which encodes an ABC transporter and is located upstream of lcrRS, in response to nukacin ISK-1. Inactivation of lctFEG significantly increased susceptibility to nukacin ISK-1. Electrophoretic mobility shift assay analysis revealed that NsrR and LcrR bound directly to regions upstream of nsrX and lctFEG, respectively. This is the first report that two distinct TCSs, NsrRS and LcrRS, are independently involved in resistance to nisin A and nukacin ISK-1 in S. mutans. Total of 14 samples were analyzed. Total RNA from each test strain and control described below were labeled with Alexa FluorÂ® 555 and Alexa FluorÂ® 647, respectively, and were cohybridized on a single array. Labeling and hybridization were performed once or twice independently. UA159 wild type as control vs. UA159 with nisinA or nukacin ISK-1, UA159 wild type with nisinA or nukacin ISK-1 vs. nsrRS or lcrRS deletion mutant in UA159 with nisinA or nukacin ISK-1.

Dataset Information

FlhC mediated gene regulation In E. coliO157:H7 on meat

Publications

Regulation of cell division, biofilm formation, and virulence by FlhC in Escherichia coli O157:H7 grown on meat.

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