Global transcriptome analysis of mouse uterus during embryo implantation by SAGE
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Zeng-Ming Yang
PROVIDER: E-GEOD-2892 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Serial analysis of gene expression in mouse uterus at the implantation site.
Ma Xing-Hong XH Hu Shi-Jun SJ Ni Hua H Zhao Yue-Chao YC Tian Zhen Z Liu Ji-Long JL Ren Gang G Liang Xiao-Huan XH Yu Hao H Wan Ping P Yang Zeng-Ming ZM
The Journal of biological chemistry 20060124 14
Although oligonucleotide chips, cDNA microarrays, differential display reverse transcription-PCR, and other approaches have been used to screen implantation-related molecules, the mechanism by which embryo implantation occurs is still unknown. The aim of this study was to profile the differential gene expression between interimplantation site and implantation site in mouse uterus on day 5 of pregnancy by serial analysis of gene expression (SAGE). In our two SAGE libraries of 11-bp tags, the tota ...[more]
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Project description:The aim of this study was to examine differential gene expression profile in mouse uterus on day 5 of pregnancy between implantation sites and inter-implantation sites. There are 104,520 tags sequenced and acquired, 51,306 for non-implantation site, and 53,214 for implantation site. Keywords: Implantation, Uterus, Differential gene expression,
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Project description:Current knowledge of the molecular regulation of the blastocyst implantation event has been largely derived from studies in the mouse that requires ovarian estrogen for initiation of the implantation event. However, there are species such as the hamster, guinea pig, pig, horse, rhesus monkey and perhaps the human where the blastocyst implantation event initiates only in the progesterone-primed uterus. Despite this fundamental difference in the requirement of ovarian hormones in initiating blastocyst implantation among species, efforts to identify gene networks relevant for the blastocyst implantation event in progesteroneM-bM-^@M-^Sdependent species are limited. In this study, cDNA prepared from RNAs of day 5 blastocyst implantation and interimplantation sites were hybridized with mouse and human oligonucleotide microarray platforms to discern the transcriptional networks underlying the regulation of blastocyst implantation in hamsters. Compared with the inter-implantation site, blastocyst implantation sites showed upregulation and downregulation of a sizable number of genes by both cross-species arrays.The merit of the cross-species hybridization and reliability of the identified up- and down-regulated genes at the implantation sites were validated by detecting differential expression of a few randomly selected genes from both arrays by real-time PCR. Function gene ontology and pathway analysis revealed that differentially expressed genes are associated with several biological events and molecular pathways that are likely to be taking place at the blastocyst implantation site. This is the first study that identified the differential gene expression profile at the blastocyst implantation site of the hamsters, and revealed molecular pathways that are possibly associated with the progesterone-dependent blastocyst implantation process. We used microarrays to detail the expression differences in hamster implantation and interimplantation sites. Total RNAs were prepared from the hamster day 5 blastocyst implantation and interimplantation sites. Three sets of RNAs were isolated from three different animals and were subjected to microarray analysis using Affymetrix mouse and human array platforms.
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