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Analysis of the gene expression pattern of the human corneal epithelial cells to prove the changing medium induces (suppresses) expression

ABSTRACT: Dr. Fukuda's laboratory is interested in determining how changing medium induces or suppresses expression of genes involved in keratan sulfte production in the corneal cells. Human corneal epithelial cells can be induced for keratan sulfate production by replacing culture medium to a serum free medium. We speculate that changing medium induces (or suppress) expression of gene involved in keratan sulfate production in the corneal cells. A microarray experiment is ideal for analyzing gene expression pattern of the cells. RNA preparations from human corneal epithelial cells under normal culture condition (control) and under induced condition for keratan sulfate production (target) were sent to the Microarray Core (E). The RNA was amplified, labeled, and hybridized to the GLYCOv3 microarrays. Data was analyzed by Core E and also provided to Dr. Fukuda's laboratory for analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Steven Head

PROVIDER: E-GEOD-28942 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analysis of the gene expression pattern of the human corneal epithelial cells to prove the changing medium induces (suppresses) expression

Project description:Dr. Fukuda's laboratory is interested in determining how changing medium induces or suppresses expression of genes involved in keratan sulfte production in the corneal cells. Human corneal epithelial cells can be induced for keratan sulfate production by replacing culture medium to a serum free medium. We speculate that changing medium induces (or suppress) expression of gene involved in keratan sulfate production in the corneal cells. A microarray experiment is ideal for analyzing gene expression pattern of the cells.

2011-04-29 | GSE28942 | GEO

Dihydrotestosterone induced changes in gene expression in primary and immortalized human corneal epithelial cells

Project description:Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in primary and immortalized human corneal epithelial cells. Analysis of regulation of primary and immortalized human corneal epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like corneal cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation and comparitive analysis of numerous genes in response to dihydrotestosterone incubation in primary and immortalized human corneal epithelial cells. Total RNA was obtained from primary and immortalized human corneal epithelial cells treated for 5 days with 10 nM dihydrotestosterone (n=3) or vehicle (n=3). The RNA was then used with Illumina HumanHT-12 v3 Expression BeadChips to determine the effect of DHT on gene expression in the primary human corneal cells grown in our laboratory and the immortalized human corneal epithelial cell line developed in Dr. Rheinwald's laboratory [Rheinwald et al. MCB, 22 (14): 5157. (2002)] and charecterized in Dr. Ilene Gibson's laboratory [Gipson et al. IOVS, 44 (6): 2496. (2003)].

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Gene expression in F9 teratocarcinoma cells

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Examine differential expression of sulf-1 and sulf-2, enzymes involved in heparan sulfate biosynthesis

Project description:Our specific aim is to examine differential expression of sulf-1 and sulf-2, enzymes involved in heparan sulfate biosynthesis, as well as Wnt ligands and Wnt signaling mediators during corneal wound healing using a mouse corneal scratch model. The specific structural features of heparan sulfate underlie its role in modulating cellular responses to growth factors such as the Wnts. Heparan sulfate 6-O-endosulfatases (sulf-1 and sulf-2) remove 6-O sulfate group from trisulfated disaccharides present on heparan sulfate chains. Our preliminary results suggest that sulf-1 is upregulated at the protein level in the epithelial cells of wounded mouse corneas, as compared to the undamaged contralateral eye. Modulation of heparan sulfate proteoglycan expression and/or structural modifications of its chains might be an important aspect of the regulation of epithelial cell migration and proliferation during wound healing. RNA preparations from four different conditions, were sent to Microarray Core (E). Gene expression was examined in triplicates at 2 time points (8 and 24 hrs post-wounding: 6 arrays), using the contralateral eye as a control at one time point as a control (3 arrays). To account for a systemic response (i.e. bilateral inflammation) to the corneal wounding, corneas of non-wounded mice (3 arrays) were used. The RNA was amplified, labeled, and hybridized to the GLYCOv3 microarrays.

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2011-04-29 | E-GEOD-28943 | biostudies-arrayexpress

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Project description:Dr. Panjwani's laboratory is focusing on the mechanism by which galectins-3 and 7 mediate corneal epithelial cell migration. We are currently performing studies to: (i) identify and characterize the corneal epithelial cell surface and extracellular matrix (ECM) molecules which serve as counterreceptors of galectin-3 and -7, to establish whether the lectins modulate corneal epithelial cell migration by binding to well known integrins, growth factor receptors, and/or ECM molecules and (ii) determine whether galectin-3 mediates corneal epithelial cell migration indirectly by modulating the expression of key adhesion and/or signal transduction molecules by using small interfering RNA, cDNA microarrays and glycogene arrays.

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Gene expression in trabecular meshwork samples from normal and glaucoma eyes

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Corneal epithelial cells

2010-12-11 | E-GEOD-26009 | biostudies-arrayexpress

Homo sapiens

Project description:Analysis of the gene expression pattern of the human corneal epithelial cells to prove the changing medium induces (suppresses) expression

| PRJNA140559 | ENA

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