Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

55 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of differential gene expression modulated by knockdown of MUC1 in MKN45 gastric carcinoma cells

ABSTRACT: Transcripts upregulated or downregulated by knockdown of MUC1 were identified through expression profiling of a total of 12,135 genes in comparison with MKN45- MUC1 RNAi clones and control clones. We endeavored to identify genes which expression is affected by MUC1 by performing cDNA microarray analysis on two MKN45 MUC1 RNAi clones and one control clone.

ORGANISM(S): Homo sapiens

SUBMITTER: Natalia Costa

PROVIDER: E-GEOD-28975 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Identification of mRNAs modulated by overexpression of N-terminal G3BP

Project description:Transcripts upregulated or downregulated by overexpression of N-terminal G3BP were identified through expression profiling of a total of 12,135 genes in comparison with S2-013 clones in which N-terminal G3BP is overexpressed and control clones. We endeavored to identify factors and pathways regulated by CD24 and to obtain leads regarding the mechanism underpinning the observed phenotypic changes by performing cDNA microarray analysis on two S2-013 CD24 RNAi clones and two control clones. Total RNAs from two clones of S2-013 CD24 RNAi or control cells were mixed and used for cDNA microarray analysis.

2010-07-31 | E-GEOD-23342 | biostudies-arrayexpress

Identification of mRNAs modulated by knockdown of CD24

Project description:We endeavored to identify factors and pathways regulated by CD24 and to obtain leads regarding the mechanism by which CD24 inhibited the invasiveness and metastasis of pancreatic cancer cells by performing cDNA microarray analysis on S2-013 CD24 RNAi clones and control clones. The group of genes showing increased expression was mainly comprised of genes for molecules with functions related to RNA metabolism, transcription factors, proteases, and molecules involved in embryonic development and cell growth. The set of genes downregulated by knockdown of CD24 included the target mRNAs of a phosphorylation-dependent endoribonuclease G3BP that interacted with CD24. The target mRNAs of G3BP were identified in GSE17056. These results suggested that CD24 inhibited the RNase activity of G3BP, and that a function of CD24 was to inhibit the degradation of specific mRNAs. Transcripts upregulated or downregulated by knockdown of CD24 were identified through expression profiling of a total of 12,135 genes in S2-013 CD24 RNAi clones compared to control clones. Before labeling with Cy5 or Cy3, total RNA from two S2-013 CD24 RNAi clones were mixed and total RNA from two control clones were mixed. The supplementary files 'GSE17055_higher_siCD24.txt' and 'GSE17055_higher_control.txt' list the differentially expressed genes.

2010-06-24 | E-GEOD-17055 | biostudies-arrayexpress

Identification of differential gene expression modulated by knockdown of MUC1 in MKN45 gastric carcinoma cells

Project description:Transcripts upregulated or downregulated by knockdown of MUC1 were identified through expression profiling of a total of 12,135 genes in comparison with MKN45- MUC1 RNAi clones and control clones.

2011-11-15 | GSE28975 | GEO

Gene expression profiling of colorectal cancers to understand molecular changes and clinicopathological features

Project description:Gene expression was analyzed in terms of canonical molecular changes and clinicopathological features to elucidate alternative or subordinate pathways during colorectal tumorigenesis and tumor growth. Eighty-four sporadic colorectal cancer patients, standardized by tumor location, were consecutively enrolled. Representative molecular changes including APC, TP53, Wnt, RAF, and mismatch repair defect (MMR) were recorded for each sample. Keywords: disease state analysis; sub-type analysis within colorectal cancers 84 samples from colorectal patients were analyzed. Paired tumor and adjacent normal tissues from the same patient were used for hybridization onto custom-made, 21k dual channel cDNA arrays. We prepared a similar number of samples from each of the three tumor locations (ascending 27, descending 29, and rectum 28) and recorded for each sample important molecular changes such as APC, TP53, RAF, WNT, and MMR mutations.

2009-02-28 | E-GEOD-10982 | biostudies-arrayexpress

Transcription profiling of Bi-sex male and female Schistosoma mansoni worms isolated from mice 7-weeks post-infection

Project description:Bi-sex male and female Schistosoma mansoni worms were isolated from mice 7-weeks post-infection. Bi-sex male material or female material was compared in a direct bimodal comparison to single-sex male or female material. Two independent biological batches of both bi- and single-sex males were used. One batch of single-sex female material was used in comparisons to three independent biological batches of bi-sex female material. A dye-swap hybridization was performed for each bimodal comparisons in turn.

2006-11-01 | E-MEXP-474 | biostudies-arrayexpress

Reconstruction evolutionary history of S. cerevisiae x S. kudriavzevii hybrids from wine beer dietetic supplement and clinical isolate

Project description:The aim of this experiment is to characterize the genome composition of the two hybrids IF6 and MR25. IF6 and MR25 are hybrids among S.cerevisiae and S. kudriavzevii. The hybridization of the genome extraction with the microarray was at 65C, under this condition only S. cerevisiae genes can hybridize. In this case we are only studying the genome composition of S. cerevisiae parental in the hybrids. The study of S. kudriavzevii parental genome was done by other methods.

2012-11-05 | E-MEXP-3375 | biostudies-arrayexpress

Methanosaeta thermophila ammonia stress

Project description:Effects of high concentration of ammonia were assessed by using mid-log phase cultures of Methanosaeta thermophila.

2013-04-01 | E-MEXP-3849 | biostudies-arrayexpress

Transcriptional profiling of Arabidopsis thaliana grown under arsenate stress

Project description:We examined the changes in gene expression in Arabidopsis thaliana grown under arsenate stress. The transcriptional profiling reveals antioxidant activity and repression of the phosphate starvation response. Keywords: dual label, stress response This experiment included a comparison of three biological replicate controls against three biological arsenate-stressed replicates with a dye-swap technical replicate for a total of six microarray slide hybridizations.

2010-05-26 | E-GEOD-10425 | biostudies-arrayexpress

Comparative genomics among S. cerevisiae x S.kudriavzevii natural hybrid strains from wine and beer reveals different origins

Project description:Genome comparison between hybrid S. cerevisiae x S. kudriavzevii strains

2011-12-31 | E-MEXP-3114 | biostudies-arrayexpress

Transcription profiling of specific organs of wheat spikes in response to Fusarium graminearum inoculation

Project description:Organ specific microarray analysis were performed to identify genes responding to Fusarium graminearum inoculation in specific organs of wheat spikes.

2007-04-25 | E-TABM-97 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data