Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression microarray for mouse ES cells vs. mouse ES cells treated with 48 hr N2B27


ABSTRACT: Core circuits of transcription factors stabilize stem and progenitor cells by suppressing genes required for differentiation. We do not know how such core circuits are reorganized during cell fate transitions to allow differentiation and lineage choice to proceed. Here, we asked how the pluripotency circuit, a core transcriptional circuit that maintains mouse embryonic stem (ES) cells in a pluripotent state, is dismantled as ES cells differentiate and choose between the neural ectodermal and mesendodermal progenitor cell fates. When ES cells are recultured from pluripotency maintaining conditions to the basal media N2B27, the expression of the pluripotency circuit genes begins to change. At 48 hours post N2B27 addition, the ES cells are competent to respond to differentiation signals. Here, our microarray analysis compares the gene expression profile of ES cells vs. the gene expression profile of cells that have been treated with N2B27 for 48 hours, reaching the competent state. 2 x mouse ES cells in pluripotency maintaining conditions. 3 x mouse ES cells after 48 hr of N2B27 culture

ORGANISM(S): Mus musculus

SUBMITTER: Matt Thomson 

PROVIDER: E-GEOD-29005 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Pluripotency factors in embryonic stem cells regulate differentiation into germ layers.

Thomson Matt M   Liu Siyuan John SJ   Zou Ling-Nan LN   Smith Zack Z   Meissner Alexander A   Ramanathan Sharad S  

Cell 20110601 6


Cell fate decisions are fundamental for development, but we do not know how transcriptional networks reorganize during the transition from a pluripotent to a differentiated cell state. Here, we asked how mouse embryonic stem cells (ESCs) leave the pluripotent state and choose between germ layer fates. By analyzing the dynamics of the transcriptional circuit that maintains pluripotency, we found that Oct4 and Sox2, proteins that maintain ESC identity, also orchestrate germ layer fate selection. O  ...[more]

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