Unknown,Transcriptomics,Genomics,Proteomics

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Microarray Expression Data from Haematopoietic Differentiated Human Embryonic Stem Cells


ABSTRACT: The underlying mechanisms which are responsible and govern early haematopoietic differentiation during development are poorly understood. Gene expression comparison between pluripotent human embryonic stem cells and earliest haematopoietic progenitors may reveal novel transcripts and pathways and provide crucial insight into early haematopoietic lineage specification and development. Understanding of transcriptional cues that direct differentiation of human embryonic stem cells (hESC) to defined and functional cell types is essential for their future clinical applications. In this study we have undertaken a comparative transcriptional approach of haematopoietic progenitors derived from hESC at various stages of a feeder and serum free differentiation method and have shown that the largest transcriptional changes occur during the first four days of differentiation. Data mining based on molecular function pointed to RhoGTPase signalling as key regulator of this differentiation. Inhibition of this pathway using a chemical inhibitor (Y26732) resulted in a significant downregulation of haematopoietic progenitors throughout the differentiation window, thus uncovering a previously unappreciated role for RhoGTPase signalling in differentiation of hESC to haematopoietic lineages. There are a total of 4 samples within this microarray experiment with 2 biological replicates for each sample. Pluripotent human embryonic stem cells (day 0) underwent haematopoietic differentiation and at various stages of development (day 4, day 6, day8) differentiated cells were FACS sorted for two key haemangioblast markers, CD31 and KDR.

ORGANISM(S): Homo sapiens

SUBMITTER: Sun Yung 

PROVIDER: E-GEOD-29115 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Large-scale transcriptional profiling and functional assays reveal important roles for Rho-GTPase signalling and SCL during haematopoietic differentiation of human embryonic stem cells.

Yung Sun S   Ledran Maria M   Moreno-Gimeno Inmaculada I   Conesa Ana A   Montaner David D   Dopazo Joaquín J   Dimmick Ian I   Slater Nicholas J NJ   Marenah Lamin L   Real Pedro J PJ   Paraskevopoulou Iliana I   Bisbal Viviana V   Burks Deborah D   Santibanez-Koref Mauro M   Moreno Ruben R   Mountford Joanne J   Menendez Pablo P   Armstrong Lyle L   Lako Majlinda M  

Human molecular genetics 20110921 24


Understanding the transcriptional cues that direct differentiation of human embryonic stem cells (hESCs) and human-induced pluripotent stem cells to defined and functional cell types is essential for future clinical applications. In this study, we have compared transcriptional profiles of haematopoietic progenitors derived from hESCs at various developmental stages of a feeder- and serum-free differentiation method and show that the largest transcriptional changes occur during the first 4 days o  ...[more]

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