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Microarray analysis of the oocyte quality

ABSTRACT: Microarray analysis was performed in ooplast samples to identify gene expression profiles associated with the embryo capacity to develop to blastocyst stage A fraction of the cytoplasm from in vitro matured oocytes was collected and snap frozen. The oocytes were chemically activated and presumptive zygotes were in vitro cultured in individual medium microdrops. The embryo development was recorded and oocytes classified according to the stage of embryo development. Oocyte was considered of good quality if the development proceeded to blastocyst stage, whereas it was considered of bad quality if there was a development block between 8-16 cells stage and morula formation.

ORGANISM(S): Bos taurus

SUBMITTER: Fernando Biase

PROVIDER: E-GEOD-29191 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Messenger RNAs in metaphase II oocytes correlate with successful embryo development to the blastocyst stage.

Biase Fernando Henrique FH Everts Robin Edward RE Oliveira Rosane R Santos-Biase Weruska Karyna Freitas WK Fonseca Merighe Giovana Krempel GK Smith Lawrence Charles LC Martelli Lúcia L Lewin Harris H Meirelles Flávio Vieira FV

Zygote (Cambridge, England) 20121010 1

The mRNAs accumulated in oocytes provide support for embryo development until embryo genomic activation. We hypothesized that the maternal mRNA stock present in bovine oocytes is associated with embryo development until the blastocyst stage. To test our hypothesis, we analyzed the transcriptome of the oocyte and correlated the results with the embryo development. Our goal was to identify genes expressed in the oocyte that correlate with its ability to develop to the blastocyst stage. A fraction ...[more]

PMID: 23046986

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