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Unknown,Transcriptomics,Genomics,Proteomics

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R.palustris: cbbT1 overexpression and cbbT2 overexpression

ABSTRACT: Transcriptional profiling of Rhodopseudomonas palustris (R. palustris) comparing cbbT1 over-expressing strain with cbbT2 over-expressing strain. Goal was to discriminate the molecular mechanisms between transketolase I (cbbT1) and transketolase II (cbbT2). R.palustris is a purple non-sulfur anoxygenic phototrophic bacterium and transketolase (cbbT1 and cbbT2) is a key enzyme involved in the CBB cycle. Here, we investogated the functions of transketolase isoforms I (cbbT1) and II (cbbT2) in R. palustris through transcriptional profiling and other functional assays. Four-condition experiment, cbbT1 over-expressing cells, cbbT2 over-expressing cells, Negative control strain with empty plasmid, Wild type strain.

ORGANISM(S): Rhodopseudomonas palustris

SUBMITTER: Chia-Wei Hu

PROVIDER: E-GEOD-29310 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Revealing the functions of the transketolase enzyme isoforms in Rhodopseudomonas palustris using a systems biology approach.

Hu Chia-Wei CW Chang Ya-Ling YL Chen Shiang Jiuun SJ Kuo-Huang Ling-Long LL Liao James C JC Huang Hsuan-Cheng HC Juan Hsueh-Fen HF

PloS one 20111208 12

<h4>Background</h4>Rhodopseudomonas palustris (R. palustris) is a purple non-sulfur anoxygenic phototrophic bacterium that belongs to the class of proteobacteria. It is capable of absorbing atmospheric carbon dioxide and converting it to biomass via the process of photosynthesis and the Calvin-Benson-Bassham (CBB) cycle. Transketolase is a key enzyme involved in the CBB cycle. Here, we reveal the functions of transketolase isoforms I and II in R. palustris using a systems biology approach.<h4>Me ...[more]

PMID: 22174789

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R.palustris: cbbT1 overexpression and cbbT2 overexpression

Project description:Transcriptional profiling of Rhodopseudomonas palustris (R. palustris) comparing cbbT1 over-expressing strain with cbbT2 over-expressing strain. Goal was to discriminate the molecular mechanisms between transketolase I (cbbT1) and transketolase II (cbbT2). R.palustris is a purple non-sulfur anoxygenic phototrophic bacterium and transketolase (cbbT1 and cbbT2) is a key enzyme involved in the CBB cycle. Here, we investogated the functions of transketolase isoforms I (cbbT1) and II (cbbT2) in R. palustris through transcriptional profiling and other functional assays.

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