Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Remote ischemic preconditioning in on-pump coronary artery bypass graft surgery

ABSTRACT: In this placebo-controlled randomized controlled trial, we tested whether remote ischemic preconditioning (RIPC) elicited by four 5-minute cycles of 300 mmHg of cuff inflation/deflation of the lower limb would reduce myocardial necrosis in isoflurane-anesthetized patients undergoing on-pump coronary artery bypass graft surgery. Secondary outcomes were the perioperative release of the biomarkers NTproBNP, hsCRP, S100, atrial transcriptional profiles, and short- and long-term clinical outcomes. RIPC with concomitantly applied isoflurane did not affect the release of biomarkers or clinical outcome. NTproBNP release correlated with isoflurane- but not RIPC-induced transcriptional changes. For eleven randomly selected patients from each group (RIPC/CTL=no RIPC) two atrial samples were collected, one at the time of cannulation (T1) and one fifteen min after releasing the cross clamp (T2). The samples were immediately frozen in liquid nitrogen and later used for RNA isolation and subsequent microarray hybridization. Gene-level analysis was performed. the results of exon-level analysis will be published separately (only preliminary results available so far).

ORGANISM(S): Homo sapiens

SUBMITTER: Eliana Lucchinetti

PROVIDER: E-GEOD-29396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Remote ischemic preconditioning applied during isoflurane inhalation provides no benefit to the myocardium of patients undergoing on-pump coronary artery bypass graft surgery: lack of synergy or evidence of antagonism in cardioprotection?

Lucchinetti Eliana E Bestmann Lukas L Feng Jianhua J Freidank Heike H Clanachan Alexander S AS Finegan Barry A BA Zaugg Michael M

Anesthesiology 20120201 2

<h4>Background</h4>Two preconditioning stimuli should induce a more consistent overall cell protection. We hypothesized that remote ischemic preconditioning (RIPC, second preconditioning stimulus) applied during isoflurane inhalation (first preconditioning stimulus) would provide more protection to the myocardium of patients undergoing on-pump coronary artery bypass grafting.<h4>Methods</h4>In this placebo-controlled randomized controlled study, patients in the RIPC group received four 5-min cyc ...[more]

PMID: 22222469

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Dataset Information

Remote ischemic preconditioning in on-pump coronary artery bypass graft surgery

Publications

Remote ischemic preconditioning applied during isoflurane inhalation provides no benefit to the myocardium of patients undergoing on-pump coronary artery bypass graft surgery: lack of synergy or evidence of antagonism in cardioprotection?

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