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Systems-wide RNAi analysis of CASP8AP2 / FLASH

ABSTRACT: We have used a combination of RNAi based functional and molecular genomic approaches to investigate in detail two proteins required for the survival of CRC cells. Set1 has 22 samples, 10 siNeg controls, 6 siRNA transfections for FLASH and NUP62 each. Set2 has 67 samples, siNeg controls at timepoint: 10hrs (5), 24hrs (6), 48hrs (6), 72hrs (5), siRNA FLASH at timepoint: 10hrs (8), 24hrs (8), 48hrs (8), 72hrs (8), untreated controls at timepoint: 10hrs (3), 24hrs (3), 48hrs (4), 72hrs (3).

ORGANISM(S): Homo sapiens

SUBMITTER: Frank Kramer

PROVIDER: E-GEOD-29405 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systems-wide RNAi analysis of CASP8AP2/FLASH shows transcriptional deregulation of the replication-dependent histone genes and extensive effects on the transcriptome of colorectal cancer cells.

Hummon Amanda B AB Pitt Jason J JJ Camps Jordi J Emons Georg G Skube Susan B SB Huppi Konrad K Jones Tamara L TL Beissbarth Tim T Kramer Frank F Grade Marian M Difilippantonio Michael J MJ Ried Thomas T Caplen Natasha J NJ

Molecular cancer 20120104

<h4>Background</h4>Colorectal carcinomas (CRC) carry massive genetic and transcriptional alterations that influence multiple cellular pathways. The study of proteins whose loss-of-function (LOF) alters the growth of CRC cells can be used to further understand the cellular processes cancer cells depend upon for survival.<h4>Results</h4>A small-scale RNAi screen of ~400 genes conducted in SW480 CRC cells identified several candidate genes as required for the viability of CRC cells, most prominentl ...[more]

PMID: 22216762

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