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Health Status, Infection and Disease in California Sea Lions (Zalophus californianus) studied using a canine microarray platform and machine-learning approaches

ABSTRACT: In this study we assessed the utility of a microarray to identify changes in gene expression predictive of health status by interrogating blood samples from California sea lions in rehabilitation. 73 California sea lion blood samples. 28 Females and 45 males. Animals were divided into 4 groups based on preliminary diagnosis at the rehabilitation center: domoic acid toxicosis (n=33, DAT), Leptospirosis infection (n=24, Lepto), control (n=4, Healthy) and other diseases (n=12, Outgroup).

ORGANISM(S): Zalophus californianus

SUBMITTER: annalaura mancia

PROVIDER: E-GEOD-29497 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Health Status, Infection and Disease in California Sea Lions (Zalophus californianus) studied using a canine microarray platform and machine-learning approaches

Project description:In this study we assessed the utility of a microarray to identify changes in gene expression predictive of health status by interrogating blood samples from California sea lions in rehabilitation.

2012-01-01 | GSE29497 | GEO

Identification of candidate protein biomarkers associated with domoic acid toxicosis in cerebrospinal fluid of California sea lions (Zalophus californianus)

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California sea lion cerebrospinal fluid LC-MS/MS

Project description:We conducted a pilot label-free LC-MS/MS study to profile and compare the cerebral spinal fluid from California sea lions with domoic acid toxicosis and without domoic acid toxicosis. CSF samples from a 8 animals with DAT and 3 controls were run on a nano LC-MS/MS (Triple TOF) and searched against a mammalian database.

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California sea lion plasma chronic DAT biomarkers via 2D-GE

Project description:Domoic acid toxicosis (DAT) in California sea lions (Zalophus californianus) is caused by exposure to the marine biotoxin domoic acid and has been linked to massive stranding events and mortality. Diagnosis is based on clinical signs in addition to the presence of domoic acid in body fluids. Chronic DAT further is characterized by reoccurring seizures progressing to status epilepticus. Diagnosis of chronic DAT is often slow and problematic, and minimally invasive tests for DAT have been the focus of numerous recent biomarker studies. The goal of this study was to retrospectively profile plasma proteins in a population of sea lions with chronic DAT and those without DAT using two dimensional gel electrophoresis in an effort to discover whether individual, multiple, or combinations of protein and clinical data could be utilized to identify sea lions with DAT. 49 spots were excised, digested and analyzed by MS/MS. These are the data.

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Identifying candidate markers of domoic acid toxicosis in undepleted plasma from California sea lions (Zalophus californianus)

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2013-01-03 | E-GEOD-34716 | biostudies-arrayexpress

Small Marine Neurotoxin Elicits Common Antibody Response in Zebrafish and Sea Lions

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