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Expression data from human CD34+ HPC subpopulations isolated from umbilical cord blood (Haddad et al. Blood 104:3918, 2004)

ABSTRACT: We used microarrays to analyze the gene expression profile of CD34+CD45RA+CD7+, CD34+CD45RA+CD10+CD19- and CD34+CD45+CD7-CD10-CD19- HPCs isolated from umbilical cord blood CD34+CD45RA+CD7+(CD10-) and CD34+CD45RA+CD10+(CD7-CD19-) HPCs correspond respectively to prothymocytes and early pre-proB precursors. CD34+CD45RA+CD7-CD10-CD19- HPCs correspond to lympho-granulo-macrophagic precursors The corresponding populations were sorted from total CD34+ HPCs isolated from 2 or 3 individual donors

ORGANISM(S): Homo sapiens

SUBMITTER: Marc Delord

PROVIDER: E-GEOD-29522 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular characterization of early human T/NK and B-lymphoid progenitor cells in umbilical cord blood.

Haddad Rima R Guardiola Philippe P Izac Brigitte B Thibault Christelle C Radich Jerry J Delezoide Anne-Lise AL Baillou Claude C Lemoine François M FM Gluckman Jean Claude JC Pflumio Françoise F Canque Bruno B

Blood 20040826 13

The early stages of human lymphopoiesis are poorly characterized. Here, we compared the lymphoid potential of a novel umbilical cord blood CD34(+)CD45RA(hi)CD7(+) hematopoietic progenitor cell (HPC) population with that of CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs, previously proposed as candidate common lymphoid progenitors. Limiting-dilution and clonal analysis, fetal thymic organ cultures, and culture onto Notch ligand Delta-like-1-expressing OP9 cells, showed that although CD34(+)CD45RA(hi)CD7(+) ...[more]

PMID: 15331438

Similar Datasets

Expression data from human CD34+ HPC subpopulations isolated from umbilical cord blood (Haddad et al. Blood 104:3918, 2004)

Project description:We used microarrays to analyze the gene expression profile of CD34+CD45RA+CD7+, CD34+CD45RA+CD10+CD19- and CD34+CD45+CD7-CD10-CD19- HPCs isolated from umbilical cord blood CD34+CD45RA+CD7+(CD10-) and CD34+CD45RA+CD10+(CD7-CD19-) HPCs correspond respectively to prothymocytes and early pre-proB precursors. CD34+CD45RA+CD7-CD10-CD19- HPCs correspond to lympho-granulo-macrophagic precursors

2011-05-25 | GSE29522 | GEO

Expression data from human CD34+ HPC subpopulations transduced with nuclear-trapped AF1q/MLLT11 (A2M)

Project description:We used microarrays to examine the impact of AF1q/MLLT11 on the gene expression profile of CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs isolated from umbilical cord blood CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs correspond respectively to early multipotent and lympho-granulo-macrophagic precursors. A2M is a nuclear mutant-derivative of AF1q/MLLT11 CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs were FACS-sorted, exposed to A2M or control vectors, sorted based on GFP expression, and subjected to global gene expression analysis 72 hrs later.

2011-05-26 | E-GEOD-29523 | biostudies-arrayexpress

Expression data from human CD34+ HPC subpopulations transduced with nuclear-trapped AF1q/MLLT11 (A2M)

2011-05-26 | GSE29523 | GEO

Functional and transcriptional heterogeneity of human hemopoietic lympho-myeloid progenitors

Project description:Cord blood (CB) samples from normal donors were obtained with informed consent. Fresh CB samples were processed within 18-34h after collection. Mononuclear cells were isolated and CD34+ fraction was separated. CB CD34+ enriched fraction was lineage depleted by staining with purified anti-human CD2, CD3, CD4, CD7, CD8a, CD11b, CD14, CD19, CD20, CD56, CD235a followed by Qdot 605 conjugated goat F(ab')2 anti-mouse IgG (H+L). Cells were also stained with anti-human CD38-FITC, CD45RA-PE or -BV650, CD123-PE Cy7, CD90-biotin, CD34- PerCP and CD10-APC. Finally, cells were incubated with streptavidin-conjugated APC-eF780 and Hoechst 33258 (Invitrogen, final concentration: 1 g/ml). Populations were defined, as follows: HSC - Lin-CD34+CD38-CD90+CD45RA-CD10-, MPP - Lin-CD34+CD38-CD90-CD45RA-CD10-, LMPP - Lin-CD34+CD38-CD90-/loCD45RA+CD10-, MLP - Lin-CD34+CD38-CD90-/loCD45RA+CD10+, GMP - Lin-CD34+CD38+CD123+CD45RA+CD10-, CMP - Lin-CD34+CD38+CD123+CD45RA-CD10-, MEP - Lin-CD34+CD38+CD123-CD45RA-CD10-.

2017-10-03 | E-MTAB-5456 | biostudies-arrayexpress

Expression data from Notch-activated CD34+CD45RA-Lin- hematopoietic progenitor cells (HPCs) transduced with nuclear-trapped AF1q/MLLT11 (A2M)

Project description:We used microarrays to examine the impact of AF1q/MLLT11 on the gene expression profile of Notch-activated CD34+CD45RA-Lin- HPCs isolated from umbilical cord blood CD34+CD45RA-Lin- HPCs correspond to early multipotent progenitors. A2M is a nuclear mutant derivative of AF1q/MLLT11 CD34+CD45RA-Lin- HPCs were FACS-sorted, exposed to A2M or control vectors, sorted again based on GFP expression, and cultured for 72 hrs with graded doses of plastic-immobilized Notch ligand Delta1ext-IgG

2011-05-26 | E-GEOD-29524 | biostudies-arrayexpress

Bulk ATAC-Seq on sorted cord blood hematopoietic populations

Project description:Bulk ATAC-Seq was performed on human cord-blood derived hematopoietis popuations using the following markers: LT-HSC; CD34+CD38-CD45RA-CD90+CD49f+ ST-HSC; CD34+CD38-CD45RA-CD90-CD49f- MLP; CD34+CD38-CD45RA+ CMP; CD34+CD38+ CD10-CD45RA-Flt3+CD71- GMP; CD34+CD38+ CD10-CD45RA+ CD71- MEP; CD34+CD38+ CD10-CD45RA-Flt3-CD71+ T cell; CD3+CD19- B cell; CD19+CD3- NK cell; CD56+CD3-CD19-CD14- Granulocyte; CD14+CD15+CD3-CD19- Monocyte; CD14+CD15-CD3-CD19- DC; CD11c+CD45+ Erythroid cell; CD71+GPA+

| EGAS00001004742 | EGA

Expression data from Notch-activated CD34+CD45RA-Lin- hematopoietic progenitor cells (HPCs) transduced with nuclear-trapped AF1q/MLLT11 (A2M)

2011-05-26 | GSE29524 | GEO

The transcriptional architecture of early human hematopoiesis identifies multilevel control of lymphoid commitment.

Project description:Here we tracked transcriptome changes over ten human hematopoietic stem and early progenitor populations, defining the transcriptional dynamics underlying the first steps of commitment. Most of the transcriptional programs observed extended beyond lineage boundaries. In particular, multi-lymphoid progenitors (MLPs) presented a hybrid transcriptional state with elements of lymphoid and myeloid programs, but also stem cell characteristics. Total RNA was obtained from flow-sorted populations of human cord blood based on the cell surface expression of CD34, CD38, CD45RA, Thy1, and CD49f, CD10, CD7, CD19 and CD1a.

2013-05-26 | E-GEOD-42414 | biostudies-arrayexpress

Genome-wide analysis of B lymphocytes derived from human pluripotent stem cells, neonatal and adult sources

Project description:The aim of the array was to compare iPS-derived CD19+CD10+ B cells with B cells from umbilical cord blood (UCB) and adult peripheral blood (PB) iPS-CD34+ cells and UCB-CD133+ cells were differentiated to the B cell lineage in vitro. B cells were also isolated directly from UCB and PB. RNA was extracted from CD19+CD10+ FACS isolated cells from all 4 B cell samples as well as from undifferentiated iPS cells.

2014-06-20 | E-GEOD-53572 | biostudies-arrayexpress

Expression data from infant ALL with MLL-AF4 rearrangement

Project description:MLL-AF4+ blasts from infant B-ALL, CB-derived CD34+CD38-CD19-CD33- HSC, CB-derived CD34+CD19+CD33- B-cell HPCs and CB-derived CD34+CD33+CD19- myeloid HPCs. We used microarray to estudy gene expression profile comparing ALL vs HSC, HPC and myeloid HPSC.

2016-03-22 | GSE79450 | GEO

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