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Genome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart

ABSTRACT: Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we sought to identify regulatory regions, gene targets and pathways regulated by this TF. To this end, we used chromatin immunoprecipitation (ChIP) of Tbx20 fused to GFP to locate binding sites in the genome of 8 weeks whole heart mouse tissue. Analysis of 1 Tbx20-GFP ChIP sample of adult mouse whole heart against whole heart input

ORGANISM(S): Mus musculus

SUBMITTER: Noboru Sakabe

PROVIDER: E-GEOD-29636 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tbx20 regulates a genetic program essential to adult mouse cardiomyocyte function.

Shen Tao T Aneas Ivy I Sakabe Noboru N Dirschinger Ralf J RJ Wang Gang G Smemo Scott S Westlund John M JM Cheng Hongqiang H Dalton Nancy N Gu Yusu Y Boogerd Cornelis J CJ Cai Chen-leng CL Peterson Kirk K Chen Ju J Nobrega Marcelo A MA Evans Sylvia M SM

The Journal of clinical investigation 20111114 12

Human mutations in or variants of TBX20 are associated with congenital heart disease, cardiomyopathy, and arrhythmias. To investigate whether cardiac disease in patients with these conditions results from an embryonic or ongoing requirement for Tbx20 in myocardium, we ablated Tbx20 specifically in adult cardiomyocytes in mice. This ablation resulted in the onset of severe cardiomyopathy accompanied by arrhythmias, with death ensuing within 1 to 2 weeks of Tbx20 ablation. Accounting for this dram ...[more]

PMID: 22080862

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Genome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart

Project description:Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we sought to identify regulatory regions, gene targets and pathways regulated by this TF. To this end, we used chromatin immunoprecipitation (ChIP) of Tbx20 fused to GFP to locate binding sites in the genome of 8 weeks whole heart mouse tissue.

2011-11-21 | GSE29636 | GEO

Genome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart

Project description:Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we generated a conditional knockout for this gene, specifically in cardiomyocytes. We profiled gene expression levels using RNA-seq in both normal and knockout adult mouse hearts to identify genes and pathways regulated by Tbx20. The article describing the Tbx20 knockout mouse is under review, a reference will be added when published. Analysis of triplicate mRNA samples of adult mouse, comparing normal and knockout

2012-02-10 | E-GEOD-30943 | biostudies-arrayexpress

ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

Project description:We performed ChIP-seq of Tbx20 tagged with GFP in mouse hearts to identify binding sites for this transcription factor. This data set is part of the study âEndocardial Tbx20 is essential for mesenchymal and myocardial cell movements required for cardiac septationâ.

2016-06-30 | E-MTAB-3967 | biostudies-arrayexpress

RNA-seq of wild-type and Tbx20 homozygous knockout E11.5 mouse cardiomyocytes to study Tbx20's roles in embryonic and adult mouse heart function

Project description:Tbx20 is a transcription factor known to play important roles in embryonic and adult mouse heart function. Our goal in this work was to better understand the function of this gene in embryonic (E11.5) mouse cardiomyocytes that form the developing chambers, expanding our knowledge of its role in heart development. To elucidate the role of Tbx20 in mouse cardiomyocytes, we generated conditional Tbx20 knockout and compared 4 samples with 4 samples of wild-type cardiomyocytes. We found evidence of regulation of cell cycle genes by Tbx20, which are involved in proliferation. In addition, Tbx20 seems to bind and regulate an enhancer of CoupTFII in the atrium, a gene involved in atrial development.

2018-06-14 | E-MTAB-5596 | biostudies-arrayexpress

Genome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart

2012-02-10 | GSE30943 | GEO

Cardiac-tbx20 overexpression enhances heart regeneration by promoting cardiomyocytes dedifferentiation and endocardial activation

Project description:The goal of this study is to understand the function of cardiac tbx20 during heart regeneration. By high-throughput sequencing, molecular variations of tbx20-cardiac specific inducing heart in response to heart injury compared with control hearts were demonstrated. We collected the injured heart apex tissue at 7 days post injury and sequenced the transcriptome.

2020-08-20 | GSE144831 | GEO

ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

Project description:ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

| PRJEB11457 | ENA

The Tbx20-TLE Interaction is Essential for the Maintenance of the Second Heart Field

Project description:T-box transcription factor 20 (Tbx20) plays a multifaceted role in cardiac morphogenesis and controls a broad gene regulatory network. However, the mechanism by which Tbx20 activates and represses target genes in a tissue-specific and temporal manner remains unclear. Studies show that Tbx20 directly interacts with the Transducin-like Enhancer of Split (TLE) family of proteins to mediate transcriptional repression. However, a function for the Tbx20-TLE transcriptional repression complex during heart development has yet to be established. We created a mouse model with a two-amino acid substitution in the Tbx20 EH1 domain, thereby disrupting the Tbx20-TLE interaction. Disruption of this interaction impaired critical morphogenic events, including cardiac looping and chamber formation. Transcriptional profiling of Tbx20EH1Mut hearts and analysis of putative direct targets revealed misexpression of the retinoic acid pathway and cardiac progenitor genes. Further, we show that altered cardiac progenitor development and function contribute to the severe cardiac defects in our model. Our studies indicate that TLE-mediated repression is a primary mechanism by which Tbx20 controls gene expression.

2023-10-03 | GSE241681 | GEO

TMT analysis of TBX20:CASZ1 mutant mice with dilated cardiomyopathy

Project description:Using an unbiased systems-based screen, we demonstrate the cardiac transcription factor TBX20 physically and genetically interacts with the essential transcription factor CASZ1. This interaction is required for survival as mice heterozygous for both Tbx20 and Casz1 die post-natally as a result of dilated cardiomyopathy (DCM).We have used quantitative proteomic approaches to define the molecular pathways mis-regulated in Tbx20 and Casz1 mice and thus, are associated with DCM. Using an unbiased systems-based screen, we demonstrate the cardiac transcription factor TBX20 physically and genetically interacts with the essential transcription factor CASZ1. This interaction is required for survival as mice heterozygous for both Tbx20 and Casz1 die post-natally as a result of dilated cardiomyopathy (DCM).We have used quantitative proteomic approaches to define the molecular pathways mis-regulated in Tbx20 and Casz1 mice and thus, are associated with DCM.

2022-05-27 | PXD005788 | Pride

Quantitative Analysis of Cardiomyocyte-specific Tbx20 Overexpression and Control Mouse Heart Transcriptomes Using Next Generation Sequencing

Project description:Purpose: The goals of this study is to determine the differential expression of transcriptome in cardiomyocyte-specific Tbx20 overexpressing adult heart (DTG) compared to controls (STG).

2018-09-02 | GSE72699 | GEO

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