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Transcription profiling of bone marrow-derived macrophages from BALB/c and C57BL/6J mice with or without IFN-gamma treatment and then infection with Yersinia enterocolitica

ABSTRACT: BMDM from BAL/c and C57BL/6 BMDM were stimlated with IFN-g overnight and infected with different Yersinia strains for 3 hrs. Experiment Overall Design: 3-4 biological replicates were analyzed from each condition

ORGANISM(S): Mus musculus

DISEASE(S): Yersinia enterocolitica WAP infected (virulent)

SUBMITTER: Reinhard Hoffmann

PROVIDER: E-GEOD-2973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Role of strain differences on host resistance and the transcriptional response of macrophages to infection with Yersinia enterocolitica.

van Erp Katrin K Dach Kristina K Koch Isabel I Heesemann Jürgen J Hoffmann Reinhard R

Physiological genomics 20051213 1

The outcome of a host-pathogen encounter is determined by virulence factors of the pathogen and defense factors of the host. We characterized the impact of host factors [resistant (C57BL/6) or susceptible (BALB/c) genetic background and exposure to interferon (IFN)-gamma] on transcriptional responses of bone marrow-derived macrophages (BMDM) to infection with Yersinia enterocolitica. IFN-gamma treatment more profoundly altered the transcriptome of BMDM than did bacterial infection or genetic bac ...[more]

PMID: 16352694

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Mouse BMDM Yersinia infection

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IFN-g counteracts YopH mediated immune evasion in Yersinia enterocolitica infection in mice

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Transcription profiling of mouse Peyers patches after infection of C57BL/6 strain with Yersinia enterocolitica

Project description:Orogastral infection of mice with Yersinia enterocolitical leads to HIF-1 alpha activation.To elucidate whether this HIF-1 alpha activation also results in a HIF-1 dependent gene programming, the transcriptomes from Peyers Patches of uninfected and Yersinia enterocolitica infected mice were analyzed by means of of microarray analyses using Affymetrix GeneChip probe arrays (MG-U74Av2). In total, 288 genes were differentially regulated three day after infection in PP compared with the expression of uninfected control mice. Of these 288 genes, 217 were found to be differentially upregulated and from these, 14 genes ( 6.5% of all upregulated genes) are well described to be regulated via HIF-1. These data indicate that orogatral infection with Y. enterocolitica results in HIF-1 dependent gene programmning Experiment Overall Design: Per group five C57BL/6 mice were infected orogastrally with 500 Million Yersinia enterocolitica. 1 and 3 days after infection, Peyers Patches were removed and total RNA was prepared. In parallel, RNA was isolated from uninfected mice. The generation of fragmented cRNA was performed following the manufacturers instructions and used for hybridization onto GeneChip arrays MG-U74Avs2. Analysis of microarray data was performed using the Affymetrix Microarray Suite 5.0, Affymetrix Mining Tool 3.0. A median signal log2 ratio (SLR) grater than 1.5 or less than -1.5 was considered a significant change.

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