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Long non-coding RNAs regulate adipogenesis (Illumina RNA-Seq)

ABSTRACT: Adipogenesis involves the regulation of hundreds of genes by several well-studied proteins, but the role of long, noncoding RNAs in this process has not been defined. We track the regulation of hundreds of lncRNAs during adipocyte differentiation, and find several that are essential for this process. We extractedbrown and white primary adipocytes and pre-adipocytes and profiled lncRNA expresssion via mRNA-Seq. We also profiled cultured, differentiated adipocytes to verify that we could recapitulate the adipocyte expression profile in preparation for a loss-of-function screen for essential adipogenic lincRNAs.

ORGANISM(S): Mus musculus

SUBMITTER: Cole Trapnell

PROVIDER: E-GEOD-29898 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Long noncoding RNAs regulate adipogenesis.

Sun Lei L Goff Loyal A LA Trapnell Cole C Alexander Ryan R Lo Kinyui Alice KA Hacisuleyman Ezgi E Sauvageau Martin M Sauvageau Martin M Tazon-Vega Barbara B Kelley David R DR Hendrickson David G DG Yuan Bingbing B Kellis Manolis M Lodish Harvey F HF Rinn John L JL

Proceedings of the National Academy of Sciences of the United States of America 20130211 9

The prevalence of obesity has led to a surge of interest in understanding the detailed mechanisms underlying adipocyte development. Many protein-coding genes, mRNAs, and microRNAs have been implicated in adipocyte development, but the global expression patterns and functional contributions of long noncoding RNA (lncRNA) during adipogenesis have not been explored. Here we profiled the transcriptome of primary brown and white adipocytes, preadipocytes, and cultured adipocytes and identified 175 ln ...[more]

PMID: 23401553

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