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Global mRNA expression profiling of HCT116 cells overexpressing miR30a-5p

ABSTRACT: Global mRNA expression profiling of HCT116 cells overexpressing miR30a-5p were collected using Agilent human whole genome array (G4845A AMADID 026652, cRNA 4x44k V2) . Two different sources of RNA were analyzed: 1.) HCT116 colon cancer cells overexpressing human mir-30a-5p together with a sponge vector expressing binding sites for the antisense sequence of sh30a-5p vector construct to compete out possible miRNAs derived from the anti-guide strand of this vector. 2.) HCT116 colon cancer cells expressing control vector (pLKO.1-LV) and the sponge vector described above. Two conditions (sponge-LV vs sponge-sh30a-5p), each condition is represented by 3 biological replicates

ORGANISM(S): Homo sapiens

SUBMITTER: Stephan Hahn 

PROVIDER: E-GEOD-29921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNAs (miRNAs) are small non-coding RNAs that are involved in different biological processes by suppressing target gene expression. Altered expression of miR-30a-5p has been reported in colon carcinoma. To elucidate its potential biological role in colon cancer, miR-30a-5p was overexpressed via a lentiviral vector system in two different colon cancer cell lines. This induced in both lines miR-30a-5p-mediated growth inhibition, attributable to a cell cycle arrest at the G(1) phase and an indu  ...[more]

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