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M-OM-^CB-dependent yabJ-spoVG operon involved in regulation of extracellular nuclease, lipase and protease expression in Staphylococcus aureus


ABSTRACT: The alternative sigma factor M-OM-^CB of Staphylococcus aureus is involved in the coordination of the general stress response, expression of virulence determinants and modulation of antibiotic resistance levels. It controls a large regulon, either directly by recognizing conserved M-OM-^CB promoter sequences, or indirectly via M-OM-^CB-dependent elements. The M-OM-^CB-controlled yabJ-spoVG operon encodes two such putative downstream elements. We report here transcriptome analysis in S. aureus Newman showing that inactivation of the yabJ-spoVG operon had primarily a repressing effect on a small subregulon comprising mainly virulence factors, including a nuclease (nuc), a protease (splE) and a lipase (lip).. As a consequence extracellular nuclease, protease and lipase activities were reduced in a yabJspoVG mutant. Trans-complementation by SpoVG was sufficient to restore their reduced phenotypic expression and lowered transcription due to yabJ-spoVG deletion. It did not restore, however, the changes triggered by M-OM-^CB inactivation, indicating that both regulons do only partially overlap, despite the M-OM-^CB dependency of the yabJ-spoVG expression. Thus, M-OM-^CB is likely to control additional, SpoVG-independent factors affecting the expression of numerous hydrolytic enzymes. SpoVG, on the other hand, seems to fine-tune the M-OM-^CB-dependent regulation of a subset of virulence factors by antagonizing the M-OM-^CB effect. Newman wild type strain compared to double mutant spoVG and yabJ or to sigB truncated mutant

ORGANISM(S): Staphylococcus aureus

SUBMITTER: FRANCOIS Patrice 

PROVIDER: E-GEOD-30115 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.

Schulthess Bettina B   Bloes Dominik A DA   François Patrice P   Girard Myriam M   Schrenzel Jacques J   Bischoff Markus M   Berger-Bächi Brigitte B  

Journal of bacteriology 20110701 18


The alternative sigma factor σ(B) of Staphylococcus aureus is involved in the coordination of the general stress response, expression of virulence determinants, and modulation of antibiotic resistance levels. It controls a large regulon, either directly by recognizing conserved σ(B) promoter sequences or indirectly via σ(B)-dependent elements. The σ(B)-controlled yabJ-spoVG operon encodes two such putative downstream elements. We report here transcriptome analysis in S. aureus Newman, showing th  ...[more]

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