Unknown,Transcriptomics,Genomics,Proteomics

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Expression data of germinating Arabidopsis seeds


ABSTRACT: In depth temporal profiling of transcript changes at 10 time points during germination in Arabidopsis seed was carried out. The time course utilised, encompassed seed maturation, stratification, germination and post-germination and provided a global investigation into the tightly regulated, phasic changes that define seed germination. A previously unidentified transient expression pattern was identified for a group of genes, whereby a significant rise in abundance was observed at the end of stratification and significantly lower expression observed up to 6 hours later. Total RNA extraction was carried out on 80 mg of Arabidopsis seeds at 10 time points during germination in triplicate. The time points selected were: freshly harvested seed (H), seeds following 15 days of ripening (0 h), seeds after; 1 h of stratification (1 h S), 12 h of stratification (12 h S), 48 h of stratification (48 h S), followed by seed collected 1 hour into the light (1 h SL), 6 hours into the light (6 h SL), 12 hours into the light (12 h SL), 24 hours into the light (24 h SL) and 48 hours into the light (48 h SL).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Simon Law 

PROVIDER: E-GEOD-30223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

In-depth temporal transcriptome profiling reveals a crucial developmental switch with roles for RNA processing and organelle metabolism that are essential for germination in Arabidopsis.

Narsai Reena R   Law Simon R SR   Carrie Chris C   Xu Lin L   Whelan James J  

Plant physiology 20110909 3


Germination represents a rapid transition from dormancy to a high level of metabolic activity. In-depth transcriptomic profiling at 10 time points in Arabidopsis (Arabidopsis thaliana), including fresh seed, ripened seed, during stratification, germination, and postgermination per se, revealed specific temporal expression patterns that to our knowledge have not previously been identified. Over 10,000 transcripts were differentially expressed during cold stratification, with subequal numbers up-r  ...[more]

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