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RNA-Seq of Sorghum bicolor 9d seedlings in response to osmotic stress and abscisic acid

ABSTRACT: This study utilized next generation sequencing technology (RNA-Seq) to examine the transcriptome of sorghum plants challenged with osmotic stress and exogenous abscisic acid (ABA) to elucidate those genes and gene networks that contribute to sorghum's tolerance to water-limiting environments with a long-term aim of developing strategies to improve plant productivity under drought. We examined the mRNA of 9 day old Sorghum bicolor (BTx623) from 2 tissue types (roots and shoots) for 2 treatments (20 uM ABA and 20% PEG) with corresponding controls (0.2M NaOH and H2O) for 27 hrs prior to harvesting, each done in triplicate biological replicates - resulting in 24 unique runs

ORGANISM(S): Sorghum bicolor

SUBMITTER: Patricia Klein

PROVIDER: E-GEOD-30249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional annotation of the transcriptome of Sorghum bicolor in response to osmotic stress and abscisic acid.

Dugas Diana V DV Monaco Marcela K MK Olsen Andrew A Klein Robert R RR Kumari Sunita S Ware Doreen D Klein Patricia E PE

BMC genomics 20111018

<h4>Background</h4>Higher plants exhibit remarkable phenotypic plasticity allowing them to adapt to an extensive range of environmental conditions. Sorghum is a cereal crop that exhibits exceptional tolerance to adverse conditions, in particular, water-limiting environments. This study utilized next generation sequencing (NGS) technology to examine the transcriptome of sorghum plants challenged with osmotic stress and exogenous abscisic acid (ABA) in order to elucidate genes and gene networks th ...[more]

PMID: 22008187

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